Cha Y S, Sachan D S
Department of Nutrition, College of Human Ecology and Agricultural Experiment Station, University of Tennessee, Knoxville 37996-1900.
J Am Coll Nutr. 1994 Aug;13(4):338-43. doi: 10.1080/07315724.1994.10718419.
The objective of this study was to determine the effects of saturated fatty acid (SFA) and unsaturated fatty acid (UFA) diets on ethanol pharmacokinetics. Hepatic ADH and plasma carnitines were also evaluated as possible indicators of the mechanism involved.
Sprague-Dawley male rats were fed modified AIN76 diets containing 10% coconut oil (SFA) or corn oil (UFA) for 120 days. A single dose (3 g/kg bw) of ethanol (13% solution) was orally administered using a gastric canula on day 30, 90, 105 and 120. Tail vein blood samples were collected at various intervals following ethanol dose and were analyzed for blood-ethanol concentration (BEC). In an analogous trial rats were given these diets for 70 days and blood samples were collected on day 35 and 63 for triglycerides, cholesterol and carnitine determination. The animals were killed on day 70 to collect liver for ADH determination.
Compared to the UFA group, the SFA group exhibited significantly higher BEC, larger area under the curve, longer half-life of ethanol, and lower rates of ethanol elimination. Plasma carnitines were also higher in the SFA vs UFA group. However, hepatic ADH activity was not different between the groups.
Dietary SFA protects liver from alcohol injury by retarding ethanol metabolism, and carnitine may be involved.
本研究的目的是确定饱和脂肪酸（SFA）和不饱和脂肪酸（UFA）饮食对乙醇药代动力学的影响。还评估了肝脏乙醇脱氢酶（ADH）和血浆肉碱作为可能涉及的机制指标。
将Sprague-Dawley雄性大鼠喂食含10%椰子油（SFA）或玉米油（UFA）的改良AIN76饮食120天。在第30、90、105和120天，使用胃插管经口给予单剂量（3 g/kg体重）乙醇（13%溶液）。在给予乙醇剂量后的不同时间间隔采集尾静脉血样，并分析血乙醇浓度（BEC）。在一项类似试验中，大鼠喂食这些饮食70天，并在第35和63天采集血样用于测定甘油三酯、胆固醇和肉碱。在第70天处死动物以采集肝脏用于ADH测定。
与UFA组相比，SFA组的BEC显著更高，曲线下面积更大，乙醇半衰期更长，乙醇消除率更低。SFA组的血浆肉碱也高于UFA组。然而，两组之间的肝脏ADH活性没有差异。
饮食中的SFA通过延缓乙醇代谢保护肝脏免受酒精损伤，肉碱可能参与其中。
