Jain Rupali, Schriever Christopher A, Danziger Larry H, Cho Sang Hyun, Rubinstein Israel
Department of Pharmacy Practice, College of Pharmacy, University of Illinois at Chicago, Chicago, IL 60612-4325, USA.
Respiration. 2007;74(3):329-33. doi: 10.1159/000101786.
A large tertiary referral hospital in inner-city Chicago.
To determine whether the IS6110 repetitive DNA element of Mycobacterium tuberculosis is detected in exhaled breath condensate of patients with newly diagnosed active pulmonary tuberculosis.
Ten hospitalized patients with positive Ziehl-Neelson-stained sputum smears were studied. Concurrent sputum cultures for mycobacteria were performed as well. Exhaled breath condensate was collected from each patient within 6 days of initiating antituberculosis chemotherapy (median 1.5 days). These samples were analyzed by polymerase chain reaction (PCR) using primers designed to amplify the IS6110 DNA fragment of M. tuberculosis. Exogenous M. tuberculosis DNA was added to exhaled breath condensate samples to detect PCR inhibitors. Concurrent cultures of exhaled breath condensate for mycobacteria were performed.
M. tuberculosis was identified in 9 of 10 sputum cultures. One isolate was identified as Mycobacterium kansasii. The IS6110 repetitive DNA element of M. tuberculosis was not detected in any of the 10 exhaled breath condensate samples. Exogenous M. tuberculosis DNA added to these samples elicited the characteristic band pattern of M. tuberculosis on agarose gel electrophoresis. No PCR inhibitors were detected. Cultures of exhaled breath condensate showed no growth of mycobacteria.
The IS6110 repetitive DNA element of M. tuberculosis is not detected in exhaled breath condensate of patients with newly diagnosed active pulmonary tuberculosis.
位于芝加哥市中心的一家大型三级转诊医院。
确定在新诊断的活动性肺结核患者的呼出气冷凝物中是否能检测到结核分枝杆菌的IS6110重复DNA元件。
对10例痰涂片抗酸染色阳性的住院患者进行研究。同时也进行了分枝杆菌痰培养。在开始抗结核化疗的6天内（中位时间1.5天）从每位患者收集呼出气冷凝物。使用设计用于扩增结核分枝杆菌IS6110 DNA片段的引物通过聚合酶链反应（PCR）对这些样本进行分析。将外源结核分枝杆菌DNA添加到呼出气冷凝物样本中以检测PCR抑制剂。同时进行呼出气冷凝物的分枝杆菌培养。
10份痰培养中有9份鉴定出结核分枝杆菌。1株分离菌鉴定为堪萨斯分枝杆菌。在10份呼出气冷凝物样本中均未检测到结核分枝杆菌的IS6110重复DNA元件。添加到这些样本中的外源结核分枝杆菌DNA在琼脂糖凝胶电泳上呈现出结核分枝杆菌的特征条带模式。未检测到PCR抑制剂。呼出气冷凝物培养未显示分枝杆菌生长。
在新诊断的活动性肺结核患者的呼出气冷凝物中未检测到结核分枝杆菌的IS6110重复DNA元件。
