el Baghdadi J, Lazraq R, Benani A, Naciri M, Ibrahimy S, Benslimane A
Unité des mycobactéries, Institut Pasteur du Maroc, Casablanca, Maroc.
Bull Soc Pathol Exot. 1997;90(5):303-6.
We have evaluated the frequency of M. tuberculosis strains which lack IS 6110 among 102 sputa isolated from Moroccan patients. A pair of primers was designed to amplify a 201bp DNA fragment of IS 6110. The amplified DNA was detected by ethidium bromide stained agarose gel electrophoresis and confirmed by southern blot hybridization with a 32P-labelled probe (PMTO2). To detect the presence of amplification inhibitors, an internal control DNA was added in each negative PCR result. Among 102 samples, 6 sputa were negative by PCR-IS 6110 but culture positive. The test of detection of M. tuberculosis for 2/6 sputa by PCR Amplicor amplifying 584 pb of rRNA 16s sequence was positive. RFLP analysis of these 2 strains revealed no bands hybridizing IS 6110 but PCR-Mt 308 was positive. These results confirmed that these M. tuberculosis strains are lacking IS 6110.
我们评估了从摩洛哥患者分离出的102份痰标本中缺乏IS 6110的结核分枝杆菌菌株的频率。设计了一对引物来扩增IS 6110的201bp DNA片段。扩增的DNA通过溴化乙锭染色的琼脂糖凝胶电泳进行检测，并用32P标记的探针（PMTO2）进行Southern印迹杂交确认。为了检测扩增抑制剂的存在，在每个阴性PCR结果中加入内部对照DNA。在102份样本中，6份痰标本经PCR-IS 6110检测为阴性，但培养结果为阳性。通过PCR Amplicor扩增16s rRNA的584 pb对2/6份痰标本进行结核分枝杆菌检测试验呈阳性。对这2株菌株的RFLP分析显示没有与IS 6110杂交的条带，但PCR-Mt 308呈阳性。这些结果证实这些结核分枝杆菌菌株缺乏IS 6110。
