Pope Georgina A, MacKenzie Donald A, Defernez Marianne, Aroso Miguel A M M, Fuller Linda J, Mellon Fred A, Dunn Warwick B, Brown Marie, Goodacre Royston, Kell Douglas B, Marvin Marcus E, Louis Edward J, Roberts Ian N
National Collection of Yeast Cultures, Institute of Food Research, Norwich Research Park, Colney, Norwich NR4 7UA, UK.
Yeast. 2007 Aug;24(8):667-79. doi: 10.1002/yea.1499.
The characterization of industrial yeast strains by examining their metabolic footprints (exometabolomes) was investigated and compared to genome-based discriminatory methods. A group of nine industrial brewing yeasts was studied by comparing their metabolic footprints, genetic fingerprints and comparative genomic hybridization profiles. Metabolic footprinting was carried out by both direct injection mass spectrometry (DIMS) and gas chromatography time-of-flight mass spectrometry (GC-TOF-MS), with data analysed by principal components analysis (PCA) and canonical variates analysis (CVA). The genomic profiles of the nine yeasts were compared by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis, genetic fingerprinting using amplified fragment length polymorphism (AFLP) analysis and microarray comparative genome hybridizations (CGH). Metabolomic and genomic analysis comparison of the nine brewing yeasts identified metabolomics as a powerful tool in separating genotypically and phenotypically similar strains. For some strains discrimination not achieved genomically was observed metabolomically.
通过检查工业酵母菌株的代谢足迹（胞外代谢组）对其进行表征，并与基于基因组的鉴别方法进行比较。通过比较九种工业酿造酵母的代谢足迹、遗传指纹图谱和比较基因组杂交图谱对其进行了研究。代谢足迹分析通过直接进样质谱法（DIMS）和气相色谱-飞行时间质谱法（GC-TOF-MS）进行，数据通过主成分分析（PCA）和典型变量分析（CVA）进行分析。通过PCR-限制性片段长度多态性（PCR-RFLP）分析、使用扩增片段长度多态性（AFLP）分析的遗传指纹图谱和微阵列比较基因组杂交（CGH）对这九种酵母的基因组图谱进行了比较。对这九种酿造酵母的代谢组学和基因组分析比较表明，代谢组学是分离基因型和表型相似菌株的有力工具。对于一些菌株，在基因组水平上未实现的鉴别在代谢组水平上被观察到。
