Pinu Farhana R, Villas-Boas Silas G
The New Zealand Institute for Plant & Food Research Limited, Private Bag 92169, Auckland 1142, New Zealand.
School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland 1010, New Zealand.
Metabolites. 2017 Aug 22;7(3):43. doi: 10.3390/metabo7030043.
Microorganisms produce and secrete many primary and secondary metabolites to the surrounding environment during their growth. Therefore, extracellular metabolites provide important information about the changes in microbial metabolism due to different environmental cues. The determination of these metabolites is also comparatively easier than the extraction and analysis of intracellular metabolites as there is no need for cell rupture. Many analytical methods are already available and have been used for the analysis of extracellular metabolites from microorganisms over the last two decades. Here, we review the applications and benefits of extracellular metabolite analysis. We also discuss different sample preparation protocols available in the literature for both types (e.g., metabolites in solution and in gas) of extracellular microbial metabolites. Lastly, we evaluate the authenticity of using extracellular metabolomics data in the metabolic modelling of different industrially important microorganisms.
微生物在生长过程中会向周围环境产生并分泌许多初级和次级代谢产物。因此，细胞外代谢产物提供了有关微生物代谢因不同环境线索而发生变化的重要信息。与细胞内代谢产物的提取和分析相比，这些代谢产物的测定相对更容易，因为无需细胞破裂。在过去二十年中，已经有许多分析方法可用于分析微生物的细胞外代谢产物。在此，我们综述了细胞外代谢产物分析的应用和益处。我们还讨论了文献中针对两种类型（例如溶液中和气体中的代谢产物）的细胞外微生物代谢产物可用的不同样品制备方案。最后，我们评估了在不同工业重要微生物的代谢建模中使用细胞外代谢组学数据的真实性。
