Suzuki Yoriyasu, Zhang Sally, Kundu Pratima, Yeung Alan C, Robbins Robert C, Yang Phillip C
Division of Cardiovascular Medicine, Stanford University, Stanford, CA 94305-5233, USA.
Magn Reson Med. 2007 Jun;57(6):1173-9. doi: 10.1002/mrm.21219.
In vivo MRI of stem cells (SCs) is an emerging application to evaluate the role of cell therapy in restoring the injured myocardium. The high spatial and temporal resolution combined with iron-oxide-based intracellular labeling techniques will provide a sensitive, noninvasive, dual imaging modality for both cells and myocardium. In order to facilitate this novel imaging approach, much effort has been directed towards developing efficient transfection methods. While techniques utilizing poly-L-lysine (PLL), protamine sulfate (PS), and electroporation (ELP) have been proposed, the fundamental biological effects of these methods on mouse embryonic SCs (mESC) have not been investigated systematically. In this study a longitudinal in vitro evaluation of cellular viability, apoptosis, proliferation, and cardiac differentiation of magnetically labeled mESC was conducted. No significant difference was seen in these biological parameters among the three transfection methods. However, cardiac differentiation was most attenuated by ELP, and iron uptake was most effective by PS.
干细胞的体内磁共振成像（MRI）是一种新兴的应用，用于评估细胞疗法在修复受损心肌中的作用。高空间和时间分辨率与基于氧化铁的细胞内标记技术相结合，将为细胞和心肌提供一种灵敏、无创的双成像模式。为了促进这种新型成像方法，人们付出了很多努力来开发高效的转染方法。虽然已经提出了利用聚-L-赖氨酸（PLL）、硫酸鱼精蛋白（PS）和电穿孔（ELP）的技术，但这些方法对小鼠胚胎干细胞（mESC）的基本生物学效应尚未得到系统研究。在本研究中，对磁性标记的mESC的细胞活力、凋亡、增殖和心脏分化进行了纵向体外评估。三种转染方法在这些生物学参数上没有显著差异。然而，电穿孔对心脏分化的抑制作用最大，而硫酸鱼精蛋白对铁摄取的效果最显著。
