Filippi Miriam, Dasen Boris, Scherberich Arnaud
Soft Robotics Laboratory, ETH Zurich, Tannenstrasse 3, 8092 Zurich, Switzerland.
Department of Biomedicine, University Hospital Basel, University of Basel, Hebelstrasse 20, 4031 Basel, Switzerland.
Materials (Basel). 2021 Aug 27;14(17):4877. doi: 10.3390/ma14174877.
By permeabilizing the cell membrane with ultrasound and facilitating the uptake of iron oxide nanoparticles, the magneto-sonoporation (MSP) technique can be used to instantaneously label transplantable cells (like stem cells) to be visualized via magnetic resonance imaging in vivo. However, the effects of MSP on cells are still largely unexplored. Here, we applied MSP to the widely applicable adipose-derived stem cells (ASCs) for the first time and investigated its effects on the biology of those cells. Upon optimization, MSP allowed us to achieve a consistent nanoparticle uptake (in the range of 10 pg/cell) and a complete membrane resealing in few minutes. Surprisingly, this treatment altered the metabolic activity of cells and induced their differentiation towards an osteoblastic profile, as demonstrated by an increased expression of osteogenic genes and morphological changes. Histological evidence of osteogenic tissue development was collected also in 3D hydrogel constructs. These results point to a novel role of MSP in remote biophysical stimulation of cells with focus application in bone tissue repair.
通过用超声使细胞膜通透化并促进氧化铁纳米颗粒的摄取，磁声孔效应（MSP）技术可用于即时标记可移植细胞（如干细胞），以便在体内通过磁共振成像进行可视化。然而，MSP对细胞的影响在很大程度上仍未得到探索。在此，我们首次将MSP应用于广泛适用的脂肪来源干细胞（ASC），并研究其对这些细胞生物学特性的影响。经过优化，MSP使我们能够在几分钟内实现一致的纳米颗粒摄取（在10 pg/细胞范围内）和完全的细胞膜重新封闭。令人惊讶的是，这种处理改变了细胞的代谢活性，并诱导它们向成骨细胞表型分化，这通过成骨基因表达的增加和形态变化得到证明。在3D水凝胶构建物中也收集到了成骨组织发育的组织学证据。这些结果表明MSP在细胞的远程生物物理刺激中具有新作用，重点应用于骨组织修复。
