Berkout Vadym D, Kryuchkov Sergey I, Doroshenko Vladimir M
MassTech, Inc., 6992 Columbia Gateway Drive, Columbia, MD 21046, USA.
Rapid Commun Mass Spectrom. 2007;21(13):2046-50. doi: 10.1002/rcm.3061.
Atmospheric pressure matrix-assisted laser desorption/ionization (AP-MALDI) has proven a convenient and rapid method for ion production in the mass spectrometric analysis of biomolecules. This technique, like other atmospheric pressure ionization methods, suffers from ion loss during ion transmission from the atmosphere into the vacuum of the mass spectrometer. In this work, a simple model describing ion formation and ion motion towards the inlet capillary of the mass spectrometer is described. Both the gas flow and electric field near the MALDI plate were numerically calculated using the boundary element method (BEM). The ions were moving along with the gas flow and drifting in the electric field in accordance with their ion mobility properties. The ion signal dependence on an electric field strength obtained in the proposed model correlates well with experimental results.
大气压基质辅助激光解吸/电离（AP-MALDI）已被证明是生物分子质谱分析中一种方便快捷的离子产生方法。与其他大气压电离方法一样，该技术在离子从大气传输到质谱仪真空区域的过程中会出现离子损失。在这项工作中，描述了一个简单的模型，该模型描述了离子的形成以及离子向质谱仪入口毛细管的运动。使用边界元法（BEM）对MALDI板附近的气流和电场进行了数值计算。离子随着气流移动，并根据其离子迁移特性在电场中漂移。在所提出的模型中获得的离子信号对电场强度的依赖性与实验结果具有良好的相关性。
