Santic Radmila, Schmidhuber Sabine M, Lang Roland, Rauch Isabella, Voglas Elena, Eberhard Nicole, Bauer Johann W, Brain Susan D, Kofler Barbara
Department of Pediatrics, University Hospital Salzburg, Paracelsus Medical University, Muellner-Hauptstrasse 48, 5020 Salzburg, Austria.
Proc Natl Acad Sci U S A. 2007 Jun 12;104(24):10217-22. doi: 10.1073/pnas.0608585104. Epub 2007 May 29.
Galanin-like peptide (GALP) is a hypothalamic neuropeptide belonging to the galanin family of peptides. The GALP gene is characterized by extensive differential splicing in a variety of murine tissues. One splice variant excludes exon 3 and results in a frame shift leading to a novel peptide sequence and a stop codon after 49 aa. In this peptide, which we termed alarin, the signal sequence of the GALP precursor peptide and the first 5 aa of the mature GALP are followed by 20 aa without homology to any other murine protein. Alarin mRNA was detected in murine brain, thymus, and skin. In accordance with its vascular localization, the peptide exhibited potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, as was also observed with other members of the galanin peptide family. However, in contrast to galanin peptides in general, the physiological effects of alarin do not appear to be mediated via the known galanin receptors. Alarin adds another facet to the surprisingly high-functional redundancy of the galanin family of peptides.
甘丙肽样肽（GALP）是一种下丘脑神经肽，属于甘丙肽肽家族。GALP基因的特征是在多种小鼠组织中存在广泛的可变剪接。一种剪接变体排除了外显子3，导致移码，从而产生一个新的肽序列，并在49个氨基酸后出现一个终止密码子。在我们称为阿拉林的这种肽中，GALP前体肽的信号序列和成熟GALP的前5个氨基酸之后是20个与任何其他小鼠蛋白质均无同源性的氨基酸。在小鼠脑、胸腺和皮肤中检测到了阿拉林mRNA。根据其血管定位，该肽在皮肤微脉管系统中表现出强效且剂量依赖性的血管收缩和抗水肿活性，甘丙肽肽家族的其他成员也观察到了这种情况。然而，与一般的甘丙肽肽不同，阿拉林的生理作用似乎不是通过已知的甘丙肽受体介导的。阿拉林为甘丙肽肽家族惊人的高功能冗余增添了新的一面。
