Jang Hyun-Seo, Kim Kyung-Ran, Choi Sang-Won, Woo Mi-Hee, Choi Jeong-Hwa
Department of Food Science and Nutrition, Catholic University of Daegu, Gyeongsan, Gyeongbuk, Korea.
Ann Nutr Metab. 2007;51(2):119-25. doi: 10.1159/000100826. Epub 2007 May 29.
This study was attempted to investigate antioxidant and antithrombus activities of water and methanol extracts of enzyme-treated Salicornia herbacea (SH)by in vitro assays observing the inhibitory activity of a rat liver microsomal lipid peroxidation, DPPH radical scavenging activity, activated partial thromboplastin times (APTT) and thromboplastin times (TT). The water and methanol extracts from enzyme-treated SH inhibited the lipid peroxidation in a dose-dependent manner over a concentration range of 0.1-1.0 mg/ml. The activity of enzyme-treated water and methanol extracts was stronger than that of non-enzyme-treated water and methanol extracts. The inhibitory activity of the water extract was higher at a concentration of 1.0 mg/ml than that of the methanol extract. The activity was the highest in the enzyme-treated water extract, and was approximately 1.08 times higher than alpha-tocopherol, a natural antioxidant. The DPPH radical scavenging activities of the SH extracts were similar to their lipid peroxidation inhibitory activity. The APTT of the water and methanol extracts was delayed at a concentration range of 0.25-2.0 mg/ml in a dose-dependent manner. The APTT of the methanol extract was longer at a concentration of 1.0 mg/ml than that of the water extracts. The enzyme-treated methanol extract exhibited the longest APTT even at a concentration of 0.50 mg/ml. The TT activities of the SH extracts were also similar to their APTT activities. These results suggest that water and methanol extracts of the enzyme-treated SH may be useful as potential antioxidant and antithrombus sources, respectively.
本研究试图通过体外试验，观察大鼠肝微粒体脂质过氧化抑制活性、DPPH自由基清除活性、活化部分凝血活酶时间（APTT）和凝血酶原时间（TT），来研究酶处理过的海蓬子（SH）水提取物和甲醇提取物的抗氧化及抗血栓活性。酶处理过的SH的水提取物和甲醇提取物在0.1 - 1.0 mg/ml浓度范围内呈剂量依赖性抑制脂质过氧化。酶处理过的水提取物和甲醇提取物的活性强于未酶处理的水提取物和甲醇提取物。水提取物在浓度为1.0 mg/ml时的抑制活性高于甲醇提取物。酶处理过的水提取物活性最高，约比天然抗氧化剂α-生育酚高1.08倍。SH提取物的DPPH自由基清除活性与其脂质过氧化抑制活性相似。水提取物和甲醇提取物在0.25 - 2.0 mg/ml浓度范围内呈剂量依赖性延长APTT。甲醇提取物在浓度为1.0 mg/ml时的APTT长于水提取物。即使在浓度为0.50 mg/ml时，酶处理过的甲醇提取物的APTT也是最长的。SH提取物的TT活性也与其APTT活性相似。这些结果表明，酶处理过的SH的水提取物和甲醇提取物可能分别作为潜在的抗氧化剂和抗血栓剂来源。
