Oh Su Kyung, Lee Nari, Cho Young Sun, Shin Dong-Bin, Choi Soon Young, Koo Minseon
Food Safety Research Division, Korea Food Research Institute, Songnam, Kyunggi-do, 463-746, Korea.
J Food Prot. 2007 May;70(5):1153-8. doi: 10.4315/0362-028x-70.5.1153.
Toxigenic Staphylococcus aureus contamination in ready-to-eat (RTE) food is a leading cause of foodborne illness in Korea. To monitor food contamination by S. aureus, a total of 3332 RTE food samples were selected from nationwide wholesale marts between 2003 and 2004 and examined. A total of 285 (8.6%) of the overall samples were contaminated by S. aureus. According to the analysis, 31.6% of the tested cream-cakes, 19.8% of the raw fish, and 19.3% of the rice cakes with filling were contaminated with S. aureus. Forty-seven percent of the strains isolated from the contaminated food were enterotoxigenic S. aureus. The phenotypic result of the strain isolated from food showed that 48% of the strains produced one or more toxins, such as staphylococcal enterotoxins A, B, and C (SEA, SEB, and SEC). At least one SEA was produced by over 90% of the toxigenic strains. Other toxins, such as SEB, SEC, SED, SEA+SEC, and SEC+SED, were each detected. Toxic shock syndrome toxin 1 (TSST-1), a causative agent of toxic shock syndrome, was detected in 13 strains of the toxigenic isolates from the food. As the result of genotyping, 22 strains with a toxin gene that was not detected in the phenotypic analysis were also detected. Sixty-nine percent of the toxigenic strains had at least one sea gene, and the most prevalent genotype was sea+seh (34.4%), followed by sea (18.8%) and sea+seg+sei (15.6%). The tst gene encoding TSST-1 was found in 13 strains (13.5%). The genes (eta and etb) encoding exfoliative toxins A and B were not detected in any of the samples.
即食（RTE）食品中的产毒素金黄色葡萄球菌污染是韩国食源性疾病的主要原因。为监测金黄色葡萄球菌对食品的污染情况，2003年至2004年期间从全国范围内的批发市场共选取了3332份即食食品样本进行检测。总体样本中共有285份（8.6%）被金黄色葡萄球菌污染。分析显示，检测的奶油蛋糕中有31.6%、生鱼中有19.8%、夹馅米糕中有19.3%被金黄色葡萄球菌污染。从受污染食品中分离出的菌株有47%是产肠毒素金黄色葡萄球菌。从食品中分离出的菌株的表型结果显示，48%的菌株产生一种或多种毒素，如葡萄球菌肠毒素A、B和C（SEA、SEB和SEC）。超过90%的产毒素菌株产生至少一种SEA。还分别检测到了其他毒素，如SEB、SEC、SED、SEA + SEC和SEC + SED。在从食品中分离出的产毒素菌株中有13株检测到了中毒性休克综合征的病原体——中毒性休克综合征毒素1（TSST - 1）。基因分型结果显示，还检测到22株在表型分析中未检测到毒素基因的菌株。69%的产毒素菌株至少有一个sea基因，最常见的基因型是sea + seh（34.4%），其次是sea（18.8%）和sea + seg + sei（15.6%）。在13株（13.5%）菌株中发现了编码TSST - 1的tst基因。在任何样本中均未检测到编码剥脱毒素A和B的基因（eta和etb）。
