土耳其马尔马拉地区食源性金黄色葡萄球菌分离株中产肠毒素葡萄球菌的流行情况、毒素基因及遗传相关性。
Prevalence of staphylococcal enterotoxins, toxin genes and genetic-relatedness of foodborne Staphylococcus aureus strains isolated in the Marmara Region of Turkey.
机构信息
Department of Food Hygiene and Technology, Faculty of Veterinary Medicine, Istanbul University, Avcilar 34320, Istanbul, Turkey.
出版信息
Int J Food Microbiol. 2011 Aug 2;148(2):99-106. doi: 10.1016/j.ijfoodmicro.2011.05.007. Epub 2011 May 14.
Staphylococcus aureus is a major foodborne pathogen and it has the ability to produce a number of extracellular toxins. We analyzed 1070 food samples obtained from retail markets and dairy farms in the Marmara Region of Turkey for the presence of S. aureus. Out of 147 isolates, 92 (62.6%) were enterotoxigenic. PCR was used to investigate the presence of staphylococcal enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq and seu), exfoliative toxin genes (eta and etb) and the toxic-shock syndrome toxin gene (tst). The PCR results showed that 53.3% of the isolates contained staphylococcal enterotoxin-like (SEl) toxin genes (seg, seh, sei, sej, sek, sel, sem, sen, seo, sep, seq and seu) which were more frequent than classical enterotoxin genes (sea to see). Furthermore, seo, sei, sem, seg, seu and sec were found in 37.0, 32.7, 30.4, 29.3, 29.3 and 27.2% of the isolates, respectively. The tst gene was detected and confirmed by DNA sequencing in 9 isolates. The presence of eta and etb were not found in the isolates. Enterotoxigenic capabilities of isolates with SEA-SEE were investigated by ELISA. Enterotoxigenic S. aureus isolates produced one to three enterotoxins, with the most frequently produced types being enterotoxin A and C. There was a correlation of 72.1% between production of a specific toxin and the presence of the respective genes. PFGE analysis was used to identify genetic-relatedness of enterotoxigenic S. aureus isolates and the results revealed that 13 groups of isolates from different or the same origin that contained the same genes showed 100% homology with indistinguishable band patterns. The other enterotoxigenic isolates showed related band patterns with 72-86% homology in sea-, 61-90% homology in sec-, 80-96% homology in seh-, and 69-96% homology in sep-positive isolates. To our knowledge, this is the first study to examine enterotoxins and related gene contents of S. aureus food isolates in the Marmara Region of Turkey.
金黄色葡萄球菌是一种主要的食源性致病菌，它能够产生多种细胞外毒素。我们分析了从土耳其马尔马拉地区的零售市场和奶牛场获得的 1070 个食品样本，以确定金黄色葡萄球菌的存在。在 147 株分离株中，有 92 株（62.6%）为肠毒素产生菌。我们使用 PCR 方法来研究葡萄球菌肠毒素基因（sea、seb、sec、sed、see、seg、seh、sei、sej、sek、sel、sem、sen、seo、sep、seq 和 seu）、表皮剥脱毒素基因（eta 和 etb）和中毒性休克综合征毒素基因（tst）的存在情况。PCR 结果显示，53.3%的分离株含有葡萄球菌肠毒素样（SEl）毒素基因（seg、seh、sei、sej、sek、sel、sem、sen、seo、sep、seq 和 seu），这比经典肠毒素基因（sea 到 see）更为常见。此外，在 37.0%、32.7%、30.4%、29.3%、29.3%和 27.2%的分离株中分别发现了 seo、sei、sem、seg、seu 和 sec。9 株分离株的 tst 基因通过 DNA 测序得到了检测和确认。在分离株中未发现 eta 和 etb。通过 ELISA 研究了具有 SEA-SEE 的分离株的肠毒素产生能力。肠毒素产生的金黄色葡萄球菌分离株产生一种至三种肠毒素，其中最常产生的类型为肠毒素 A 和 C。特定毒素的产生与相应基因的存在之间存在 72.1%的相关性。PFGE 分析用于鉴定肠毒素产生的金黄色葡萄球菌分离株的遗传相关性，结果表明，来自不同或相同来源的 13 组含有相同基因的分离株显示出 100%的同源性，且条带模式无法区分。其他肠毒素产生的分离株在 sea- 中显示出 72-86%同源性的相关条带模式，在 sec- 中显示出 61-90%同源性，在 seh- 中显示出 80-96%同源性，在 sep-阳性分离株中显示出 69-96%同源性。据我们所知，这是首次在土耳其马尔马拉地区研究金黄色葡萄球菌食品分离株的肠毒素和相关基因含量。
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