Rundle A, Madsen A, Orjuela M, Mooney L, Tang D, Kim M, Perera F
Department of Epidemiology, Mailman School of Public Health, 722 West 168th Street, 7th floor, New York, NY 10032, USA.
Biomarkers. 2007 Mar-Apr;12(2):123-32. doi: 10.1080/13547500601010418.
Prior work suggests that body size and fat content may influence carcinogen-DNA adduct levels measured in white blood cells. Here we consider energy balance more broadly by assessing the impact of body mass index (BMI), physical activity and calorie intake on the presence of benzo[a]pyrene-DNA (BP-DNA) adducts in white blood cell DNA. Our cross-sectional study employed subjects from a separately conducted intervention trial. Physical activity and food intake data were collected at 12 and 15 months of follow-up, respectively. BP-DNA adducts were measured by high-performance liquid chromatography (HPLC) in white blood cell samples collected at 12 months of follow-up. Complete data on all variables were available from 143 subjects. Logistic regression showed that BMI was inversely associated with the presence of detectable adducts (OR = 0.90, p = 0.02), and that hours of moderate-intensity physical activity were positively associated with the presence of detectable adducts (OR = 1.04, p = 0.04). These results provide further evidence that body fat content influences carcinogen-DNA adduct levels, probably by altering the distribution of the lipophilic parent compound.
先前的研究表明，体型和脂肪含量可能会影响在白细胞中测得的致癌物 - DNA加合物水平。在此，我们通过评估体重指数（BMI）、身体活动和卡路里摄入量对白细胞DNA中苯并[a]芘 - DNA（BP - DNA）加合物存在情况的影响，更广泛地考虑能量平衡。我们的横断面研究采用了来自另一项单独进行的干预试验的受试者。身体活动和食物摄入数据分别在随访的第12个月和第15个月收集。通过高效液相色谱法（HPLC）在随访第12个月收集的白细胞样本中测量BP - DNA加合物。143名受试者可获得所有变量的完整数据。逻辑回归显示，BMI与可检测到的加合物的存在呈负相关（OR = 0.90，p = 0.02），中度强度身体活动的时长与可检测到的加合物的存在呈正相关（OR = 1.04，p = 0.04）。这些结果提供了进一步的证据，表明体脂含量可能通过改变亲脂性母体化合物的分布来影响致癌物 - DNA加合物水平。
