Magnani Rodrigo F, De Souza Gezimar D, Rodrigues-Filho Edson
Laboratório de Espectrometria de Massas, Departamento de Química, Universidade Federal de São Carlos-UFSCar, Rodovia Washington Luiz, São Carlos, São Paulo, Brazil.
J Agric Food Chem. 2007 Jun 27;55(13):4980-6. doi: 10.1021/jf0704256. Epub 2007 May 31.
A method was developed for the quantification of alternariol and alternariol monomethyl ether on tangerines with and without symptoms of Alternaria brown spot disease. The method employs solid-phase extraction for cleanup, followed by high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS) for detection. This method was validated on flavedo (exocarp or epicarp, exterior yellow peel) and on albedo tissue (mesocarp, interior white peel). An excellent linearity over a range of 0.50-20.0 mg/kg was achieved, with r2 >or= 0.997. The limits of detection (LOD) and quantification (LOQ) were fewer than 0.13 and 0.50 microg/kg, respectively. The relative standard deviations (RSDs) were <or=14.4% during the validation. The levels of these mycotoxins on flavedo of fruits with symptoms vary from 2.54 +/- 0.24 to 17.40 +/- 1.05 mug/kg. Surprisingly, neither alternariol nor altenariol monomethyl ether was detected on albedo tissues, suggesting that flavedo works as a barrier for such substances.
开发了一种用于定量分析有和没有链格孢褐斑病症状的柑橘上的交链孢酚和交链孢酚单甲醚的方法。该方法采用固相萃取进行净化，随后通过高效液相色谱-串联质谱法（HPLC-MS/MS）进行检测。此方法在柑橘外果皮（外果皮或表皮，外部黄色果皮）和内果皮组织（中果皮，内部白色果皮）上得到验证。在0.50 - 20.0 mg/kg范围内实现了出色的线性，r2≥0.997。检测限（LOD）和定量限（LOQ）分别低于0.13和0.50 μg/kg。验证期间相对标准偏差（RSD）≤14.4%。有症状果实的外果皮上这些霉菌毒素的含量在2.54±0.24至17.40±1.05 μg/kg之间。令人惊讶的是，在内果皮组织上未检测到交链孢酚和交链孢酚单甲醚，这表明外果皮对这些物质起到了屏障作用。
