Chen Jimin M, Willers Craig, Xu Jiake, Wang Allan, Zheng Ming-Hao
Department of Orthopaedic Surgery, School of Surgery and Pathology, University of Western Australia, Nedlands, Australia.
Tissue Eng. 2007 Jul;13(7):1479-91. doi: 10.1089/ten.2006.0266.
Large and retracted rotator cuff tendon tears fail to repair or retear after surgical intervention. This study attempted to develop novel tissue-engineering approaches using tenocyte-seeded bioscaffolds for tendon reconstruction of massive rotator cuff tendon defect in rabbits. Porcine small intestine submucosa (Restore) and type I/III collagen bioscaffold (ACI-Maix) were chosen as bioscaffold carriers for autologous tenocytes. Biological characterization of autologous tenocytes was conducted before the implantation. The tenocyte-seeded bioscaffolds were implanted as interposition grafts to reconstruct massive rotator cuff tendon defects in rabbits. In situ reimplantation of the autologous rotator cuff tendon, excised during defect creation, served as a positive control. Histological outcomes were analyzed and semi-quantitatively graded at 4 and 8 weeks after surgery. At 4 weeks, both tenocyte-seeded bioscaffolds displayed inflammatory reaction similar to bioscaffold-only cuff reconstruction, and the histological grading were inferior to control repair. However, at 8 weeks, inflammatory reaction of both tenocyte-seeded bioscaffolds were dramatically less than with bioscaffold alone. In addition, bioscaffolds seeded with tenocytes generated a histological appearance similar to that of the positive control. The implantation of autologous tenocytes on collagen-based bioscaffolds results in better rotator cuff tendon healing and remodeling than with the implantation of bioscaffold alone.
大型回缩性肩袖肌腱撕裂在手术干预后无法修复或再次撕裂。本研究试图开发新的组织工程方法，使用接种了肌腱细胞的生物支架来重建兔巨大肩袖肌腱缺损。选择猪小肠黏膜下层（Restore）和I/III型胶原生物支架（ACI-Maix）作为自体肌腱细胞的生物支架载体。在植入前对自体肌腱细胞进行生物学特性分析。将接种了肌腱细胞的生物支架作为间置移植物植入，以重建兔的巨大肩袖肌腱缺损。在创建缺损时切除的自体肩袖肌腱原位再植入作为阳性对照。在术后4周和8周分析组织学结果并进行半定量分级。在4周时，两种接种了肌腱细胞的生物支架均表现出与仅使用生物支架的肩袖重建相似的炎症反应，并且组织学分级低于对照修复。然而，在8周时，两种接种了肌腱细胞的生物支架的炎症反应明显低于仅使用生物支架的情况。此外，接种了肌腱细胞的生物支架产生的组织学外观与阳性对照相似。与仅植入生物支架相比，在基于胶原的生物支架上植入自体肌腱细胞可导致更好的肩袖肌腱愈合和重塑。
