Jakobsen Jette, Maribo Hanne, Bysted Anette, Sommer Helle M, Hels Ole
National Food Institute, Technical University of Denmark, Mørkhøj Bygade 19, DK-2860 Søborg, Denmark.
Br J Nutr. 2007 Nov;98(5):908-13. doi: 10.1017/S0007114507756933. Epub 2007 May 31.
In food databases, the specific contents of vitamin D3 and 25-hydroxyvitamin D3 in food have been implemented in the last 10 years. No consensus has yet been established on the relative activity between the components. Therefore, the objective of the present study was to assess the relative activity of 25-hydroxyvitamin D3 compared to vitamin D3. The design was a parallel study in pigs (n 24), which from an age of 12 weeks until slaughter 11 weeks later were fed approximately 55 microg vitamin D/d, as vitamin D3, in a mixture of vitamin D3 and 25-hydroxyvitamin D3, or 25-hydroxyvitamin D3. The end-points measured were plasma 25-hydroxyvitamin D3, and in the liver and loin the content of vitamin D3 and 25-hydroxyvitamin D3. Vitamin D3 and 25-hydroxyvitamin D3 in the feed did not affect 25-hydroxyvitamin D3 in the plasma, liver or loin differently, while a significant effect was shown on vitamin D3 in the liver and loin (P < 0.001). 25-Hydroxyvitamin D3 in the plasma, liver and loin significantly correlates with the sum of vitamin D3 and 25-hydroxyvitamin D3 in the feed (P < 0.05). Therefore, 25-hydroxyvitamin D3 should be regarded as having the same activity as vitamin D3 in food databases. Sole use of 25-hydroxyvitamin D3 as a vitamin D source in pig feed will produce liver and meat with a negligible content of vitamin D3, while an increased content of vitamin D3 in the feed will produce liver and meat with increased content of both vitamin D3 and 25-hydroxyvitamin D3.
在食品数据库中，食品中维生素D3和25-羟基维生素D3的具体含量在过去10年中已得到应用。目前尚未就这些成分之间的相对活性达成共识。因此，本研究的目的是评估25-羟基维生素D3相对于维生素D3的相对活性。实验设计为对猪进行平行研究（n = 24），从12周龄开始，直到11周后屠宰，每天以维生素D3、维生素D3和25-羟基维生素D3的混合物或25-羟基维生素D3的形式，给猪喂食约55微克维生素D。测量的终点指标为血浆25-羟基维生素D3，以及肝脏和腰部肌肉中维生素D3和25-羟基维生素D3的含量。饲料中的维生素D3和25-羟基维生素D3对血浆、肝脏或腰部肌肉中的25-羟基维生素D3没有不同影响，但对肝脏和腰部肌肉中的维生素D3有显著影响（P < 0.001）。血浆、肝脏和腰部肌肉中的25-羟基维生素D3与饲料中维生素D3和25-羟基维生素D3的总量显著相关（P < 0.05）。因此，在食品数据库中，25-羟基维生素D3应被视为与维生素D3具有相同活性。在猪饲料中仅使用25-羟基维生素D3作为维生素D来源，会使肝脏和肉类中维生素D3的含量可忽略不计，而饲料中维生素D3含量的增加会使肝脏和肉类中维生素D3和25-羟基维生素D3的含量都增加。
