Selvam Ramasamy, Ganesan Kalaivani, Narayana Raju K V S, Gangadharan Akkalayi Chandrapuram, Manohar Bhakthavatchalam Murali, Puvanakrishnan Rengarajulu
Department of Pharmacology and Toxicology, Madras Veterinary College, Vepery, Chennai, India.
Life Sci. 2007 Jun 6;80(26):2403-10. doi: 10.1016/j.lfs.2007.03.019. Epub 2007 May 1.
Rheumatoid arthritis (RA) is a chronic inflammatory disorder affecting 1% of the population worldwide. Pulsed electromagnetic field (PEMF) has a number of well-documented physiological effects on cells and tissues including antiinflammatory effect. This study aims to explore the antiinflammatory effect of PEMF and its possible mechanism of action in amelioration of adjuvant induced arthritis (AIA). Arthritis was induced by a single intradermal injection of heat killed Mycobacterium tuberculosis at a concentration of 500 microg in 0.1 ml of paraffin oil into the right hind paw of rats. The arthritic animals showed a biphasic response regarding changes in the paw edema volume. During the chronic phase of the disease, arthritic animals showed an elevated level of lipid peroxides and depletion of antioxidant enzymes with significant radiological and histological changes. Besides, plasma membrane Ca(2+) ATPase (PMCA) activity was inhibited while intracellular Ca(2+) level as well as prostaglandin E(2) levels was noticed to be elevated in blood lymphocytes of arthritic rats. Exposure of arthritic rats to PEMF at 5 Hzx4 microT x 90 min, produced significant antiexudative effect resulting in the restoration of the altered parameters. The antiinflammatory effect could be partially mediated through the stabilizing action of PEMF on membranes as reflected by the restoration of PMCA and intracellular Ca(2+) levels in blood lymphocytes subsequently inhibiting PGE(2) biosynthesis. The results of this study indicated that PEMF could be developed as a potential therapy for RA in human beings.
类风湿性关节炎（RA）是一种慢性炎症性疾病，影响着全球1%的人口。脉冲电磁场（PEMF）对细胞和组织具有许多有充分文献记载的生理效应，包括抗炎作用。本研究旨在探讨PEMF的抗炎作用及其在改善佐剂诱导性关节炎（AIA）中的可能作用机制。通过在大鼠右后爪皮内单次注射浓度为500微克/0.1毫升石蜡油中的热灭活结核分枝杆菌来诱导关节炎。关节炎动物在爪水肿体积变化方面表现出双相反应。在疾病的慢性期，关节炎动物的脂质过氧化物水平升高，抗氧化酶耗竭，伴有明显的放射学和组织学变化。此外，关节炎大鼠血淋巴细胞中的质膜Ca(2+)ATP酶（PMCA）活性受到抑制，而细胞内Ca(2+)水平以及前列腺素E(2)水平则升高。将关节炎大鼠暴露于5Hzx4微特斯拉x90分钟的PEMF下，产生了显著的抗渗出作用，导致改变的参数恢复。抗炎作用可能部分通过PEMF对膜的稳定作用介导，这表现为血淋巴细胞中PMCA和细胞内Ca(2+)水平的恢复，随后抑制PGE(2)生物合成。本研究结果表明，PEMF可开发成为人类RA的一种潜在治疗方法。
