Chang Kyle, Chang Walter Hong-Shong
Department of Biomedical Engineering, Chung-Yuan Christian University, Chung-Li, Taiwan, Republic of China.
Bioelectromagnetics. 2003 Apr;24(3):189-98. doi: 10.1002/bem.10078.
With the use of Helmholtz coils and pulsed electromagnetic field (PEMF) stimulators to generate uniform time varying electromagnetic fields, the effects of extremely low frequency electromagnetic fields on osteoporosis and serum prostaglandin E(2) (PGE(2)) concentration were investigated in bilaterally ovariectomized rats. Thirty-five 3 month old female Sprague-Dawley rats were randomly divided into five different groups: intact (INT), ovariectomy (OVX), aspirin treated (ASP), PEMF stimulation (PEMF + OVX), and PEMF stimulation with aspirin (PEMF + ASP) groups. All rats were subjected to bilateral ovariectomy except those in INT group. Histomorphometric analyses showed that PEMF stimulation augmented and restored proximal tibial metaphyseal trabecular bone mass (increased hard tissue percentage, bone volume percentage, and trabecular number) and architecture (increased trabecular perimeter, trabecular thickness, and decreased trabecular separation) in both PEMF + OVX and PEMF + ASP. Trabecular bone mass of PEMF + OVX rats after PEMF stimulation for 30 days was restored to levels of age matched INT rats. PEMF exposure also attenuated the higher serum PGE(2) concentrations of OVX rats and restored it to levels of INT rats. These experiments demonstrated that extremely low intensity, low frequency, single pulse electromagnetic fields significantly suppressed the trabecular bone loss and restored the trabecular bone structure in bilateral ovariectomized rats. We, therefore, conclude that PEMF may be useful in the prevention of osteoporosis resulting from ovariectomy and that PGE(2) might relate to these preventive effects.
利用亥姆霍兹线圈和脉冲电磁场（PEMF）刺激器产生均匀随时间变化的电磁场，在双侧卵巢切除的大鼠中研究了极低频电磁场对骨质疏松症和血清前列腺素E2（PGE2）浓度的影响。35只3个月大的雌性Sprague-Dawley大鼠被随机分为五组：完整组（INT）、卵巢切除组（OVX）、阿司匹林治疗组（ASP）、PEMF刺激组（PEMF + OVX）和PEMF刺激联合阿司匹林组（PEMF + ASP）。除INT组外，所有大鼠均接受双侧卵巢切除术。组织形态计量学分析表明，PEMF刺激增加并恢复了PEMF + OVX和PEMF + ASP组胫骨近端干骺端小梁骨量（硬组织百分比、骨体积百分比和小梁数量增加）和结构（小梁周长、小梁厚度增加，小梁间距减小）。PEMF刺激30天后，PEMF + OVX大鼠的小梁骨量恢复到年龄匹配的INT组大鼠水平。PEMF暴露还降低了OVX大鼠较高的血清PGE2浓度，并将其恢复到INT组大鼠水平。这些实验表明，极低强度、低频、单脉冲电磁场显著抑制双侧卵巢切除大鼠的小梁骨丢失并恢复小梁骨结构。因此，我们得出结论，PEMF可能有助于预防卵巢切除引起的骨质疏松症，且PGE2可能与这些预防作用有关。
