Pournajafi-Nazarloo Hossein, Perry Adam, Partoo Leila, Papademeteriou Eros, Azizi Fereidoun, Carter C Sue, Cushing Bruce S
Brain-Body Center, Department of Psychiatry, College of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.
Peptides. 2007 Jun;28(6):1170-7. doi: 10.1016/j.peptides.2007.04.022. Epub 2007 May 6.
Oxytocin (OT) has been implicated in reproductive functions, induction of maternal behavior as well as endocrine and neuroendocrine regulation of the cardiovascular system. Here we demonstrate that neonatal manipulation of OT can modulate the mRNAs expression for OT receptor (OTR), atrial natriuretic peptide (ANP), endothelial nitric oxide synthase (eNOS) and estrogen receptor alpha (ERalpha) in the heart. On the first day of postnatal life, female and male rats were randomly assigned to receive one of the following treatments: (a) 50microl i.p. injection of 7microg OT; (b) 0.7microg of OT antagonist (OTA); or (c) isotonic saline (SAL). Hearts were collected either on postnatal day 1 or day 21 (D1 or D21) and the mRNAs expression of OTR, ANP, inducible NOS (iNOS), eNOS, ERalpha and estrogen receptor beta (ERbeta) were compared by age, treatment, and sex utilizing real time PCR. OT treatment significantly increased heart OTR, ANP and eNOS mRNAs expression on D1 in both males and females, ERalpha increased only in females. While there were significant changes in the relative expression of all types of mRNA between D1 and D21, there were no significant treatment effects observed in D21 animals. OTA treatment significantly decreased basal ANP and eNOS mRNAs expression on D1 in both sexes. The results indicate that during the early postnatal period OT can have an immediate effect on the expression OTR, ANP, eNOS, and ERalpha mRNAs and that these effects are mitigated by D21. Also with the exception of ERalpha mRNA, the effects are the same in both sexes.
催产素（OT）参与生殖功能、母性行为的诱导以及心血管系统的内分泌和神经内分泌调节。在此，我们证明新生儿期对OT的调控可调节心脏中OT受体（OTR）、心房利钠肽（ANP）、内皮型一氧化氮合酶（eNOS）和雌激素受体α（ERα）的mRNA表达。在出生后的第一天，将雌性和雄性大鼠随机分为以下处理组之一：（a）腹腔注射50微升含7微克OT的溶液；（b）0.7微克OT拮抗剂（OTA）；或（c）等渗盐水（SAL）。在出生后第1天或第21天（D1或D21）采集心脏，利用实时PCR按年龄、处理方式和性别比较OTR、ANP、诱导型一氧化氮合酶（iNOS）、eNOS、ERα和雌激素受体β（ERβ）的mRNA表达。OT处理显著增加了D1时雄性和雌性大鼠心脏中OTR、ANP和eNOS的mRNA表达，ERα仅在雌性大鼠中增加。虽然D1和D21之间所有类型mRNA的相对表达有显著变化，但在D21的动物中未观察到显著的处理效应。OTA处理显著降低了D1时两性的基础ANP和eNOS的mRNA表达。结果表明，在出生后早期，OT可对OTR、ANP、eNOS和ERα的mRNA表达产生即时影响，且这些影响在D21时减弱。此外，除ERα mRNA外，两性的影响相同。
