Loffredo John T, Maxwell Jess, Qi Ying, Glidden Chrystal E, Borchardt Gretta J, Soma Taeko, Bean Alex T, Beal Dominic R, Wilson Nancy A, Rehrauer William M, Lifson Jeffrey D, Carrington Mary, Watkins David I
Wisconsin National Primate Research Center, University of Wisconsin-Madison, 555 Science Drive, Madison, WI 53711, USA.
J Virol. 2007 Aug;81(16):8827-32. doi: 10.1128/JVI.00895-07. Epub 2007 May 30.
Certain major histocompatibility complex (MHC) class I alleles are associated with the control of human immunodeficiency virus and simian immunodeficiency virus (SIV) replication. We have designed sequence-specific primers for detection of the rhesus macaque MHC class I allele Mamu-B08 by PCR and screened a cohort of SIV-infected macaques for this allele. Analysis of 196 SIV(mac)239-infected Indian rhesus macaques revealed that Mamu-B08 was significantly overrepresented in elite controllers; 38% of elite controllers were Mamu-B08 positive compared to 3% of progressors (P = 0.00001). Mamu-B08 was also associated with a 7.34-fold decrease in chronic phase viremia (P = 0.002). Mamu-B*08-positive macaques may, therefore, provide a good model to understand the correlates of MHC class I allele-associated immune protection and viral containment in human elite controllers.
某些主要组织相容性复合体（MHC）I类等位基因与人类免疫缺陷病毒和猿猴免疫缺陷病毒（SIV）复制的控制相关。我们设计了序列特异性引物，通过聚合酶链反应（PCR）检测恒河猴MHC I类等位基因Mamu - B08，并对一组感染SIV的猕猴进行该等位基因的筛查。对196只感染SIV（mac）239的印度恒河猴的分析显示，Mamu - B08在精英控制者中显著富集；38%的精英控制者Mamu - B08呈阳性，而疾病进展者中这一比例为3%（P = 0.00001）。Mamu - B08还与慢性期病毒血症降低7.34倍相关（P = 0.002）。因此，Mamu - B*08阳性的猕猴可能为理解人类精英控制者中MHC I类等位基因相关免疫保护和病毒控制的关联因素提供一个良好的模型。
