监测α干扰素对单个细胞的抗病毒作用。
Monitoring the antiviral effect of alpha interferon on individual cells.
作者信息
Kim Chon Saeng, Jung Jong Ha, Wakita Takaji, Yoon Seung Kew, Jang Sung Key
机构信息
PBC, Department of Life Science, Pohang University of Science and Technology, San31, Hyoja-Dong, Pohang 790-784, Republic of Korea.
出版信息
J Virol. 2007 Aug;81(16):8814-20. doi: 10.1128/JVI.02824-06. Epub 2007 May 30.
Abstract
An infectious hepatitis C virus (HCV) cDNA clone (JFH1) was generated recently. However, quantitative analysis of HCV infection and observation of infected cells have proved to be difficult because the yield of HCV in cell cultures is fairly low. We generated infectious HCV clones containing the convenient reporters green fluorescent protein (GFP) and Renilla luciferase in the NS5a-coding sequence. The new viruses responded to antiviral agents in a dose-dependent manner. Responses of individual cells containing HCV to alpha interferon (IFN-alpha) were monitored using GFP-tagged HCV and time-lapse confocal microscopy. Marked variations in the response to IFN-alpha were observed among HCV-containing cells.
摘要
一种传染性丙型肝炎病毒（HCV）cDNA克隆（JFH1）最近被构建出来。然而，由于HCV在细胞培养中的产量相当低，对HCV感染进行定量分析以及观察受感染细胞已被证明是困难的。我们构建了在NS5a编码序列中含有方便的报告基因绿色荧光蛋白（GFP）和海肾荧光素酶的传染性HCV克隆。新病毒对抗病毒药物呈剂量依赖性反应。使用GFP标记的HCV和延时共聚焦显微镜监测单个含有HCV的细胞对α干扰素（IFN-α）的反应。在含有HCV的细胞中观察到对IFN-α反应的显著差异。
相似文献
1
Monitoring the antiviral effect of alpha interferon on individual cells.监测α干扰素对单个细胞的抗病毒作用。
J Virol. 2007 Aug;81(16):8814-20. doi: 10.1128/JVI.02824-06. Epub 2007 May 30.
2
A novel luciferase and GFP dual reporter virus for rapid and convenient evaluation of hepatitis C virus replication.一种新型的荧光素酶和 GFP 双报告病毒，用于快速方便地评估丙型肝炎病毒复制。
Virus Res. 2011 Feb;155(2):406-14. doi: 10.1016/j.virusres.2010.11.009. Epub 2010 Dec 7.
3
Development of a simple system for screening anti-hepatitis C virus drugs utilizing mutants capable of vigorous replication.开发一种利用能够大量复制的突变体筛选抗丙型肝炎病毒药物的简单系统。
J Virol Methods. 2010 Nov;169(2):380-4. doi: 10.1016/j.jviromet.2010.08.009. Epub 2010 Aug 14.
4
A recombinant replication-competent hepatitis C virus expressing Azami-Green, a bright green-emitting fluorescent protein, suitable for visualization of infected cells.一种表达阿萨米绿（一种发出亮绿色荧光的蛋白质）的具有复制能力的重组丙型肝炎病毒，适用于可视化感染细胞。
Biochem Biophys Res Commun. 2008 Dec 5;377(1):7-11. doi: 10.1016/j.bbrc.2008.08.145. Epub 2008 Sep 9.
5
Mycophenolic acid augments interferon-stimulated gene expression and inhibits hepatitis C Virus infection in vitro and in vivo.霉酚酸增强干扰素刺激基因表达并抑制体内外丙型肝炎病毒感染。
Hepatology. 2012 Jun;55(6):1673-83. doi: 10.1002/hep.25562. Epub 2012 Apr 18.
6
Multiple effects of Honokiol on the life cycle of hepatitis C virus.和厚朴酚对丙型肝炎病毒生命周期的多种影响。
Liver Int. 2012 Jul;32(6):989-97. doi: 10.1111/j.1478-3231.2011.02621.x. Epub 2011 Aug 11.
7
Development of a robust luciferase reporter 1b/2a hepatitis C virus (HCV) for characterization of early stage HCV life cycle inhibitors.开发一种稳健的荧光素酶报告 1b/2a 丙型肝炎病毒（HCV），用于表征 HCV 生命周期抑制剂的早期阶段。
Antiviral Res. 2013 Apr;98(1):85-92. doi: 10.1016/j.antiviral.2013.01.005. Epub 2013 Feb 1.
8
Inhibition of intracellular hepatitis C virus replication by nelfinavir and synergistic effect with interferon-alpha.奈非那韦对细胞内丙型肝炎病毒复制的抑制作用及与α干扰素的协同效应。
J Viral Hepat. 2009 Jul;16(7):506-12. doi: 10.1111/j.1365-2893.2009.01102.x. Epub 2009 Mar 3.
9
Hepatitis C virus replication is inhibited by 22beta-methoxyolean-12-ene-3beta, 24(4beta)-diol (ME3738) through enhancing interferon-beta.22β-甲氧基齐墩果-12-烯-3β,24(4β)-二醇（ME3738）通过增强β-干扰素抑制丙型肝炎病毒复制。
Hepatology. 2008 Jul;48(1):59-69. doi: 10.1002/hep.22289.
10
Mizoribine inhibits hepatitis C virus RNA replication: effect of combination with interferon-alpha.咪唑立宾抑制丙型肝炎病毒RNA复制：与α干扰素联合使用的效果
Biochem Biophys Res Commun. 2005 May 13;330(3):871-9. doi: 10.1016/j.bbrc.2005.03.062.
引用本文的文献
1
Adapted HCV JFH1 variant is capable of accommodating a large foreign gene insert and allows lower level HCV replication and viral production.适应型 HCV JFH1 变体能够容纳较大的外源基因插入，并允许较低水平的 HCV 复制和病毒产生。
Int J Biol Sci. 2018 Jul 13;14(10):1211-1220. doi: 10.7150/ijbs.27411. eCollection 2018.
2
The Intracellular Cholesterol Transport Inhibitor U18666A Inhibits the Exosome-Dependent Release of Mature Hepatitis C Virus.细胞内胆固醇转运抑制剂U18666A抑制成熟丙型肝炎病毒的外泌体依赖性释放。
J Virol. 2016 Nov 28;90(24):11181-11196. doi: 10.1128/JVI.01053-16. Print 2016 Dec 15.
3
A Dual-reporter system for real-time monitoring and high-throughput CRISPR/Cas9 library screening of the hepatitis C virus.一种用于丙型肝炎病毒实时监测和高通量CRISPR/Cas9文库筛选的双报告系统。
Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038/srep08865.
4
Direct visualization of hepatitis C virus-infected Huh7.5 cells with a high titre of infectious chimeric JFH1-EGFP reporter virus in three-dimensional Matrigel cell cultures.在三维 Matrigel 细胞培养物中，用高滴度感染性嵌合 JFH1-EGFP 报告病毒直接观察到丙型肝炎病毒感染的 Huh7.5 细胞。
J Gen Virol. 2014 Feb;95(Pt 2):423-433. doi: 10.1099/vir.0.055772-0. Epub 2013 Nov 16.
5
Functional characterization of bovine viral diarrhea virus nonstructural protein 5A by reverse genetic analysis and live cell imaging.通过反向遗传分析和活细胞成像对牛病毒性腹泻病毒非结构蛋白5A进行功能表征
J Virol. 2014 Jan;88(1):82-98. doi: 10.1128/JVI.01957-13. Epub 2013 Oct 16.
6
Discovery of cellular proteins required for the early steps of HCV infection using integrative genomics.利用整合基因组学发现 HCV 感染早期所需的细胞蛋白。
PLoS One. 2013 Apr 12;8(4):e60333. doi: 10.1371/journal.pone.0060333. Print 2013.
7
Development of a flow cytometry live cell assay for the screening of inhibitors of hepatitis C virus (HCV) replication.
Open Virol J. 2012;6:97-102. doi: 10.2174/1874357901206010097. Epub 2012 Nov 26.
8
Hepatitis C variability, patterns of resistance, and impact on therapy.丙型肝炎的变异性、耐药模式及其对治疗的影响。
Adv Virol. 2012;2012:267483. doi: 10.1155/2012/267483. Epub 2012 Jul 19.
9
Generation of a cell culture-adapted hepatitis C virus with longer half life at physiological temperature.在生理温度下生成半衰期更长的细胞培养适应型丙型肝炎病毒。
PLoS One. 2011;6(8):e22808. doi: 10.1371/journal.pone.0022808. Epub 2011 Aug 4.
10
Hepatitis C virus infection is blocked by HMGB1 released from virus-infected cells.丙型肝炎病毒感染被病毒感染细胞释放的高迁移率族蛋白 B1 所阻断。
J Virol. 2011 Sep;85(18):9359-68. doi: 10.1128/JVI.00682-11. Epub 2011 Jul 13.
本文引用的文献
1
Characterization of the early steps of hepatitis C virus infection by using luciferase reporter viruses.利用荧光素酶报告病毒对丙型肝炎病毒感染早期步骤的表征
J Virol. 2006 Jun;80(11):5308-20. doi: 10.1128/JVI.02460-05.
2
Subcellular localization of hepatitis C virus structural proteins in a cell culture system that efficiently replicates the virus.丙型肝炎病毒结构蛋白在高效复制该病毒的细胞培养系统中的亚细胞定位。
J Virol. 2006 Mar;80(6):2832-41. doi: 10.1128/JVI.80.6.2832-2841.2006.
3
Time- and temperature-dependent activation of hepatitis C virus for low-pH-triggered entry.丙型肝炎病毒在低pH值触发进入时的时间和温度依赖性激活
J Virol. 2006 Feb;80(4):1734-41. doi: 10.1128/JVI.80.4.1734-1741.2006.
4
Efficient hepatitis C virus cell culture system: what a difference the host cell makes.高效丙型肝炎病毒细胞培养系统：宿主细胞的作用差异巨大。
Proc Natl Acad Sci U S A. 2005 Jul 12;102(28):9739-40. doi: 10.1073/pnas.0504296102. Epub 2005 Jul 5.
5
Production of infectious hepatitis C virus in tissue culture from a cloned viral genome.从克隆的病毒基因组在组织培养中产生传染性丙型肝炎病毒。
Nat Med. 2005 Jul;11(7):791-6. doi: 10.1038/nm1268. Epub 2005 Jun 12.
6
Complete replication of hepatitis C virus in cell culture.丙型肝炎病毒在细胞培养中的完全复制。
Science. 2005 Jul 22;309(5734):623-6. doi: 10.1126/science.1114016. Epub 2005 Jun 9.
7
Robust hepatitis C virus infection in vitro.体外强大的丙型肝炎病毒感染
Proc Natl Acad Sci U S A. 2005 Jun 28;102(26):9294-9. doi: 10.1073/pnas.0503596102. Epub 2005 Jun 6.
8
Sequestration of TRAF2 into stress granules interrupts tumor necrosis factor signaling under stress conditions.在应激条件下，TRAF2隔离于应激颗粒中会中断肿瘤坏死因子信号传导。
Mol Cell Biol. 2005 Mar;25(6):2450-62. doi: 10.1128/MCB.25.6.2450-2462.2005.
9
Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes.将绿色荧光蛋白插入非结构蛋白5A可直接观察功能性丙型肝炎病毒复制复合体。
J Virol. 2004 Jul;78(14):7400-9. doi: 10.1128/JVI.78.14.7400-7409.2004.
10
An NS3 protease inhibitor with antiviral effects in humans infected with hepatitis C virus.一种对丙型肝炎病毒感染的人类具有抗病毒作用的NS3蛋白酶抑制剂。
Nature. 2003 Nov 13;426(6963):186-9. doi: 10.1038/nature02099. Epub 2003 Oct 26.
Zotero 插件