Gorby G L, Clemens C M, Barley L R, McGee Z A
Section of Infectious Diseases, Omaha VA Medical Center, NE 68105.
Microb Pathog. 1991 May;10(5):373-84. doi: 10.1016/0882-4010(91)90082-l.
The possible effect of human chorionic gonadotropin (hCG) on the mucosal immune response and susceptibility of the fallopian tube mucosa to invasion by Neisseria gonorrhoeae (gonococci) was investigated in the fallopian tube organ culture (FTOC) model. Immunohistochemical and radioreceptor assay techniques showed specific high affinity binding of hCG in vitro to the apices of non-ciliated fallopian tube cells (Kd approximately 10(-9) M). Continuous exposure of the FTOC mucosa to hCG during infection with gonococci resulted in a marked increase (6- to 15-fold) in IgA secretion and significantly reduced gonococcal invasion (invasion score range 0.7 to 1.75) compared to infected control tissue which was not exposed to hCG (invasion score range 2.9 to 4.95, P less than or equal to 0.01). By contrast, exposure of the mucosa to hCG during the 24 h preceding gonococcal infection followed by the removal of hCG from the system at the time of infection resulted in enhanced gonococcal invasion (invasion score range 7.95 to 9.7, P less than 0.001). We conclude that hCG can modulate the mucosal immune response and susceptibility of fallopian tube epithelium to gonococcal invasion.
在输卵管器官培养（FTOC）模型中，研究了人绒毛膜促性腺激素（hCG）对黏膜免疫反应以及输卵管黏膜对淋病奈瑟菌（淋球菌）侵袭易感性的可能影响。免疫组织化学和放射受体分析技术显示，hCG在体外与输卵管非纤毛细胞顶端具有特异性高亲和力结合（解离常数约为10^(-9) M）。与未暴露于hCG的感染对照组织（侵袭评分范围为2.9至4.95，P≤0.01）相比，在淋球菌感染期间FTOC黏膜持续暴露于hCG导致IgA分泌显著增加（6至15倍），且淋球菌侵袭明显减少（侵袭评分范围为0.7至1.75）。相比之下，在淋球菌感染前24小时将黏膜暴露于hCG，然后在感染时从系统中去除hCG，会导致淋球菌侵袭增强（侵袭评分范围为7.95至9.7，P＜0.001）。我们得出结论，hCG可调节黏膜免疫反应以及输卵管上皮对淋球菌侵袭的易感性。
