Haak-Frendscho M, Kurtz R S, Czuprynski C J
Department of Pathobiological Sciences, School of Veterinary Medicine, Madison, Wisconsin 53706.
Microb Pathog. 1991 May;10(5):385-92. doi: 10.1016/0882-4010(91)90083-m.
We have previously demonstrated that administration of recombinant rIL-1 alpha enhances resistance against Listeria monocytogenes infection in mice. In this study we considered the possibility that this cytokine might also augment adoptive immunity conferred by the transfer of listeria-immune spleen cells. Concomitant administration of rIL-1 alpha with large numbers (2 x 10(7) or 10(8)) of listeria-immune spleen cells reduced the protection mediated by the transferred cells. Conversely, rIL-1 alpha co-administered with suboptimal numbers (1-5 x 10(6)) of immune splenocytes augmented anti-listeria resistance in an additive fashion. Although transfer of 10(6) listeria-immune spleen cells alone did not result in significant protection, when 10(6) immune cells were incubated with rIL-1 alpha prior to transfer they conferred significant protection to naive recipients. Time course experiments indicated that the greatest protection was achieved when listeria-immune spleen cells were pretreated with rIL-1 alpha for 2 h prior to adoptive transfer. The protection transferred by 10(6) rIL-1 alpha-pretreated immune spleen cells was not inhibited by TGF beta. This study is the first to use rIL-1 alpha to potentiate the adoptive transfer of resistance to an infectious agent by immune cells.
我们先前已证明，给予重组rIL-1α可增强小鼠对单核细胞增生李斯特菌感染的抵抗力。在本研究中，我们考虑了这种细胞因子可能还会增强由李斯特菌免疫脾细胞转移所赋予的过继免疫的可能性。将rIL-1α与大量（2×10⁷或10⁸）李斯特菌免疫脾细胞同时给予，会降低转移细胞介导的保护作用。相反，将rIL-1α与次优数量（1 - 5×10⁶）的免疫脾细胞共同给予时，会以累加方式增强抗李斯特菌的抵抗力。虽然单独转移10⁶个李斯特菌免疫脾细胞不会产生显著的保护作用，但当10⁶个免疫细胞在转移前与rIL-1α孵育时，它们会给未免疫的受体提供显著的保护。时间进程实验表明，当过继转移前用rIL-1α预处理李斯特菌免疫脾细胞2小时时，可实现最大程度的保护。10⁶个经rIL-1α预处理的免疫脾细胞所转移的保护作用不受转化生长因子β的抑制。本研究首次使用rIL-1α来增强免疫细胞对感染因子抵抗力的过继转移。
