Czuprynski C J, Brown J F, Young K M, Cooley A J, Kurtz R S
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison 53706.
J Immunol. 1988 Feb 1;140(3):962-8.
The effects of exogenously administered rIL-1 alpha on elimination of viable listeriae from the liver and spleen during the course of a primary Listeria monocytogenes infection was studied. Similar numbers of L. monocytogenes were recovered from rIL-1 alpha-treated and control mice at up to 24 h after infection; however, by 48 h after infection more than 1 log10 fewer viable L. monocytogenes were recovered from the spleens of rIL-1 alpha-treated mice than from Listeria-infected controls. The difference in bacterial burden between IL-1 alpha-treated and control mice increased with time; by 7 days after infection viable L. monocytogenes had been eliminated from most rIL-1 alpha-treated mice, whereas control mice still harbored 10(4) to 10(5) L. monocytogenes per spleen and liver. Histopathologic examination confirmed that rIL-1 alpha-treated mice suffered considerably less damage to the spleen, liver, lung, and brain than did control mice. To determine whether rIL-1 alpha-mediated protection indirectly by augmenting the release of other cytokines, we determined serum levels of colony-stimulating activity and IFN activity in rIL-1 alpha-treated and control Listeria-infected mice. Treatment with rIL-alpha elicited an early burst of serum colony-stimulating activity as compared with sera from Listeria-infected control mice. These data suggest that exogenous administration of rIL-1 initiates release of colony-stimulating activity, and perhaps other cytokines, that accelerate the protective response of the infected host. Prophylactic augmentation of antimicrobial resistance by administration of rIL-1 alpha may be worthy of further evaluation.
研究了外源性给予重组白细胞介素-1α（rIL-1α）对原发性单核细胞增生李斯特菌感染过程中肝脏和脾脏内活菌清除的影响。在感染后长达24小时内，从rIL-1α处理组和对照组小鼠中回收的单核细胞增生李斯特菌数量相似；然而，在感染后48小时，与感染李斯特菌的对照组相比，从rIL-1α处理组小鼠脾脏中回收的活菌数量减少了超过1个对数10。IL-1α处理组和对照组小鼠之间的细菌负荷差异随时间增加；感染后7天，大多数rIL-1α处理组小鼠体内的活菌单核细胞增生李斯特菌已被清除，而对照组小鼠每个脾脏和肝脏中仍含有10⁴至10⁵个单核细胞增生李斯特菌。组织病理学检查证实，rIL-1α处理组小鼠脾脏、肝脏、肺和脑的损伤程度明显低于对照组小鼠。为了确定rIL-1α是否通过增强其他细胞因子的释放间接发挥保护作用，我们测定了rIL-1α处理组和感染李斯特菌的对照组小鼠血清中的集落刺激活性和干扰素活性水平。与感染李斯特菌的对照组小鼠血清相比，rIL-α处理引发了血清集落刺激活性的早期爆发。这些数据表明，外源性给予rIL-1可引发集落刺激活性以及可能其他细胞因子的释放，从而加速感染宿主的保护反应。通过给予rIL-1α预防性增强抗菌抗性可能值得进一步评估。
