Han Chang-Kyun, Choi Won Rack, Oh Ki-Bong
Life Science R&D Center, SK Chemicals, Changan-gu, Suwon-si, Gyeonggi-do, Korea.
Planta Med. 2007 Jun;73(7):665-9. doi: 10.1055/s-2007-981530. Epub 2007 May 31.
The root extract of Pulsatilla koreana (Ranunculaceae) has been found to have prominent abilities to reverse scopolamine-induced cognitive impairment in rats, and to increase the viability of human neuroblastoma SK-N-SH cells incubated with amyloid-beta peptide (1 - 42) [A beta (1 - 42)]. In vivo and in vitro activity-guided fractionation studies using solvent-partitioning and subsequent chromatographic separations led to the isolation of hederacolchiside-E, an oleanolic glycoside, as an active ingredient. Administration of hederacolchiside-E (30 and 60 mg/kg body weight, P. O.) increased the step-through latency time in the passive avoidance test as efficiently as tacrine (30 mg/kg, P. O.). The neuroprotective effect of hederacolchiside-E on SK-N-SH cells against the toxicity of A beta (1 - 42) was comparable to that of catechin. These data suggest that hederacolchiside-E might be a good therapeutic candidate for the treatment of Alzheimer's disease.
朝鲜白头翁（毛茛科）的根提取物已被发现具有显著能力，可逆转东莨菪碱诱导的大鼠认知障碍，并提高与β-淀粉样肽（1-42）[Aβ（1-42）]共同孵育的人神经母细胞瘤SK-N-SH细胞的活力。使用溶剂分配和随后的色谱分离进行的体内和体外活性导向分级分离研究，导致分离出一种齐墩果烷型糖苷——常春藤皂苷元E，作为活性成分。给予常春藤皂苷元E（30和60mg/kg体重，口服）在被动回避试验中增加穿梭潜伏期的效果与他克林（30mg/kg，口服）一样有效。常春藤皂苷元E对SK-N-SH细胞抵抗Aβ（1-42）毒性的神经保护作用与儿茶素相当。这些数据表明，常春藤皂苷元E可能是治疗阿尔茨海默病的良好候选药物。
