OBJECTIVE

To prepare chitosan coated puerarin liposomes and investigate their physicochemical properties.

METHODS

Puerarin liposomes were prepared by reverse phase evaporation technique and then coated with chitosan. Using encapsulation efficiency as index of examination and designing an orthogonal experiment, the optimal formulation of liposomes was determined. The physicochemical properties of uncoatd and chitosan coated puerarin liposomes were investigated, respectively.

RESULTS

Uncoated and chitosan coated puerarin liposomes were spherelike and smooth. The mean particles size of uncoated and coated liposomes were 217. 3nm and 632. 6nm, respectively. The Zeta potential were -14.44 mV and +35.61 mV, respectively. The encapsulating efficency was 50. 6% and 51.1%, respectively.

CONCLUSION

Puerarin liposomes can be prepared by reverse phase evaporation technique successfully. The chitosan coated purarin liposomes ware spherelike and smooth.