[高效毛细管电泳法测定苦参碱脂质体中苦参碱的含量]
[HPCE determination of matrine in matrine liposome].
作者信息
Zhang Xiu-ming, Liu Xiao-ping, Li Hui
机构信息
Central Station of Chinese Medicinal Materials Information, Station Food and Drug Administration, Guangzhou 510080, China.
出版信息
Zhong Yao Cai. 2007 Jan;30(1):97-9.
OBJECTIVE
To establish an HPCE method for the determination of matrine in Matrine Liposome.
METHODS
HPCE was used. The best condition was achieved with a fused silica capillary tube( 150 cm x 75 microm), 0.25 mol/L phosphate buffer (pH 6.8), at constant voltage of 20 kV and temperature at 25 degrees C. The UV detection wavelength was 206 nm.
RESULTS
The calibration curve was linear in the range of 0.3-6.025 mg/ml for matrine (0.9995). The average recovery of matrine was 97.3%.
CONCLUSION
This methods is simple, quick, accurate and suitable for the quality control for Matrine Liposome.
目的
建立一种用于测定苦参碱脂质体中苦参碱含量的高效毛细管电泳（HPCE）方法。
方法
采用高效毛细管电泳法。使用熔融石英毛细管柱（150 cm×75μm），0.25 mol/L磷酸盐缓冲液（pH 6.8），在20 kV恒压和25℃温度条件下进行测定。紫外检测波长为206 nm。
结果
苦参碱在0.3 - 6.025 mg/ml范围内校准曲线呈线性（0.9995）。苦参碱的平均回收率为97.3%。
结论
该方法简便、快速、准确，适用于苦参碱脂质体的质量控制。
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