Garay-Arroyo A, Covarrubias A A, Clark I, Niño I, Gosset G, Martinez A
Departamento de Biología Molecular de Plantas, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Apdo. Postal 510-3, 62250, Cuernavaca, Morelos, Mexico.
Appl Microbiol Biotechnol. 2004 Feb;63(6):734-41. doi: 10.1007/s00253-003-1414-4. Epub 2003 Aug 9.
Two sets of Saccharomyces cerevisiae strains were compared for their physiological responses to different stress conditions. One group is composed of three strains adapted to controlled laboratory conditions (CEN.PK, LR88 and RS58), whereas the other consisted of five industrial strains (IND1101, SuperStart, LO24, LO41 and Azteca). Most industrial strains showed higher tolerance to heat shock and to an oxidative environment than laboratory strains. Excluding CEN.PK, a similar behavior was observed regarding ethanol production in high sugar concentrations (180 g/l glucose). Addition of acetate (10 g/l) or furfural (2 g/l), in concentrations similar to those found in sugar cane bagasse hydrolysates, decreased cell mass formation and growth rate in almost all strains. CEN.PK and SuperStart showed the highest sensitivity when grown in furfural-containing medium. Acetic acid treatment severely affected cell mass formation and reduced growth rate in all strains; CEN.PK and LO24 were the most resistant. The specific ethanol production rate was not affected by furfural addition. However, specific ethanol production rates decreased in response to acetic acid in four industrial strains, and increased in all laboratory strains and in LO24. No significant correlation was found between the stress tolerance of the strains tested and the transcript accumulation of genes selected by their involvement in the response to each of the stressful environments applied.
比较了两组酿酒酵母菌株对不同胁迫条件的生理反应。一组由三种适应实验室控制条件的菌株组成（CEN.PK、LR88和RS58），而另一组由五种工业菌株组成（IND1101、SuperStart、LO24、LO41和Azteca）。大多数工业菌株对热休克和氧化环境的耐受性高于实验室菌株。除CEN.PK外，在高糖浓度（180 g/l葡萄糖）下乙醇生产方面观察到类似行为。添加浓度与甘蔗渣水解物中相似的乙酸（10 g/l）或糠醛（2 g/l），几乎降低了所有菌株的细胞质量形成和生长速率。在含糠醛的培养基中生长时，CEN.PK和SuperStart表现出最高的敏感性。乙酸处理严重影响所有菌株的细胞质量形成并降低生长速率；CEN.PK和LO24最具抗性。添加糠醛不影响比乙醇生产率。然而，四种工业菌株的比乙醇生产率因乙酸而降低，所有实验室菌株和LO24中的比乙醇生产率则升高。在所测试菌株的胁迫耐受性与其参与应对每种施加的应激环境而选择的基因的转录积累之间未发现显著相关性。
