Kakudo Natsuko, Shimotsuma Ayuko, Kusumoto Kenji
Department of Plastic and Reconstructive Surgery, Kansai Medical University, 10-15 Fumizono, Moriguchi, Osaka, Japan.
Biochem Biophys Res Commun. 2007 Jul 27;359(2):239-44. doi: 10.1016/j.bbrc.2007.05.070. Epub 2007 May 21.
Adipose-derived stem cells (ASCs) have demonstrated a capacity for differentiating into a variety of lineages, including bone, cartilage, or fat, depending on the inducing stimuli and specific growth and factors. It is acknowledged that fibroblast growth factor-2 (FGF-2) promotes chondrogenic and inhibits osteogenic differentiation of ASCs, but thorough investigations of its effects on adipogenic differentiation are lacking. In this study, we demonstrate at the cellular and molecular levels the effect of FGF-2 on adipogenic differentiation of ASCs, as induced by an adipogenic hormonal cocktail consisting of 3-isobutyl-1-methylxanthine (IBMX), dexamethasone, insulin, and indomethacin. FGF-2 significantly enhances the adipogenic differentiation of human ASCs. Furthermore, in cultures receiving FGF-2 before adipogenic induction, mRNA expression of peroxisome proliferator-activated receptor gamma2 (PPARgamma2), a key transcription factor in adipogenesis, was upregulated. The results of FGF-2 supplementation suggest the potential applications of FGF-2 and ASCs in adipose tissue regeneration.
脂肪来源干细胞（ASCs）已显示出能够分化为多种细胞谱系，包括骨、软骨或脂肪，这取决于诱导刺激以及特定的生长因子。众所周知，成纤维细胞生长因子2（FGF-2）可促进ASCs的软骨形成并抑制其成骨分化，但目前缺乏对其在脂肪生成分化方面作用的深入研究。在本研究中，我们在细胞和分子水平上证明了FGF-2对由3-异丁基-1-甲基黄嘌呤（IBMX）、地塞米松、胰岛素和吲哚美辛组成的脂肪生成激素混合物诱导的ASCs脂肪生成分化的影响。FGF-2显著增强了人ASCs的脂肪生成分化。此外，在脂肪生成诱导前接受FGF-2的培养物中，脂肪生成关键转录因子过氧化物酶体增殖物激活受体γ2（PPARγ2）的mRNA表达上调。补充FGF-2的结果表明FGF-2和ASCs在脂肪组织再生中的潜在应用。
