Wilson Jonathan M, Sato Kazuna, Chernoff Ellen A G, Belecky-Adams Teri L
Department of Biology and Center for Regenerative Biology and Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA.
Gene Expr Patterns. 2007 Aug;7(7):817-25. doi: 10.1016/j.modgep.2007.04.001. Epub 2007 Apr 21.
Vertebrate homologues of musashi have recently been referred to as neural stem cell markers because of their expression patterns and RNA-binding interactions. In the context of the notch signaling pathway, Musashi-1 (Msi-1) is a regulator of neural cell generation, cooperating with notch to maintain mitosis. In an effort to identify definitive stem cell markers of the neural retina, a portion of the Msi-1 cDNA was cloned, and the expression of Msi-1 in the chick eye was analyzed. Using an Msi-1-specific antibody and RNA probe, we show that expression of Msi-1 in the early neural tube is consistent with neural stem identity. In the neural retina, expression starts shortly before embryonic day 3 (E3) and continues up to and including E18. A BrdU incorporation assay shows Msi-1 to be found in both proliferating and differentiating cells of E5 neural retina. At E8 (when proliferation is complete in the fundus of the retina) and E18 (mature retina) Msi-1 expression was found in the ciliary marginal zone (CMZ) as well as in a subpopulation of differentiated cells, including photoreceptors and ganglion cells.
由于其表达模式和RNA结合相互作用，musashi的脊椎动物同源物最近被称为神经干细胞标志物。在Notch信号通路的背景下，Musashi-1（Msi-1）是神经细胞生成的调节因子，与Notch协同维持有丝分裂。为了鉴定神经视网膜的确定性干细胞标志物，克隆了一部分Msi-1 cDNA，并分析了Msi-1在鸡眼中的表达。使用Msi-1特异性抗体和RNA探针，我们发现Msi-1在早期神经管中的表达与神经干细胞身份一致。在神经视网膜中，表达在胚胎第3天（E3）前不久开始，并持续到E18，包括E18。BrdU掺入试验表明，在E5神经视网膜的增殖细胞和分化细胞中都发现了Msi-1。在E8（视网膜底部增殖完成时）和E18（成熟视网膜）时，在睫状边缘区（CMZ）以及包括光感受器和神经节细胞在内的一部分分化细胞中发现了Msi-1表达。
