Taura Futoshi, Sirikantaramas Supaart, Shoyama Yoshinari, Yoshikai Kazuyoshi, Shoyama Yukihiro, Morimoto Satoshi
Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka 812-8582, Japan.
FEBS Lett. 2007 Jun 26;581(16):2929-34. doi: 10.1016/j.febslet.2007.05.043. Epub 2007 May 25.
Cannabidiolic-acid (CBDA) synthase is the enzyme that catalyzes oxidative cyclization of cannabigerolic-acid into CBDA, the dominant cannabinoid constituent of the fiber-type Cannabis sativa. We cloned a novel cDNA encoding CBDA synthase by reverse transcription and polymerase chain reactions with degenerate and gene-specific primers. Biochemical characterization of the recombinant enzyme demonstrated that CBDA synthase is a covalently flavinylated oxidase. The structural and functional properties of CBDA synthase are quite similar to those of tetrahydrocannabinolic-acid (THCA) synthase, which is responsible for the biosynthesis of THCA, the major cannabinoid in drug-type Cannabis plants.
大麻二酚酸（CBDA）合酶是一种将大麻二酚酸氧化环化生成CBDA的酶，CBDA是纤维型大麻中主要的大麻素成分。我们使用简并引物和基因特异性引物，通过逆转录和聚合酶链反应克隆了一个编码CBDA合酶的新cDNA。重组酶的生化特性表明，CBDA合酶是一种共价结合黄素的氧化酶。CBDA合酶的结构和功能特性与四氢大麻酚酸（THCA）合酶非常相似，THCA合酶负责药物型大麻植物中主要大麻素THCA的生物合成。
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