Onofri Chiara, de Meijer Etienne P M, Mandolino Giuseppe
Consiglio per la ricerca in agricoltura e l'analisi dell'economia agraria, Centro di Ricerca per le Colture Industriali, via di Corticella 133, 40128 Bologna, Italy.
GW Pharmaceuticals PLC, Ground Floor South Wing, Kingsgate House, Newbury Road, Andover SP10 4DU, United Kingdom.
Phytochemistry. 2015 Aug;116:57-68. doi: 10.1016/j.phytochem.2015.03.006. Epub 2015 Apr 9.
Sequence variants of THCA- and CBDA-synthases were isolated from different Cannabis sativa L. strains expressing various wild-type and mutant chemical phenotypes (chemotypes). Expressed and complete sequences were obtained from mature inflorescences. Each strain was shown to have a different specificity and/or ability to convert the precursor CBGA into CBDA and/or THCA type products. The comparison of the expressed sequences led to the identification of different mutations, all of them due to SNPs. These SNPs were found to relate to the cannabinoid composition of the inflorescence at maturity and are therefore proposed to have a functional significance. The amount of variation was found to be higher within the CBDAS sequence family than in the THCAS family, suggesting a more recent evolution of THCA-forming enzymes from the CBDAS group. We therefore consider CBDAS as the ancestral type of these synthases.
从表达各种野生型和突变化学表型（化学类型）的不同大麻品种中分离出四氢大麻酸（THCA）和大麻二酚酸（CBDA）合酶的序列变体。从成熟的花序中获得了表达的完整序列。结果表明，每个品种将前体大麻酸（CBGA）转化为CBDA和/或THCA型产物的特异性和/或能力各不相同。对表达序列的比较导致鉴定出不同的突变，所有这些突变均由单核苷酸多态性（SNP）引起。发现这些SNP与成熟花序中的大麻素组成有关，因此被认为具有功能意义。结果发现，CBDAS序列家族中的变异量高于THCAS家族，这表明来自CBDAS组的THCA形成酶的进化更为近期。因此，我们认为CBDAS是这些合酶的原始类型。
