Oregon CBD, Independence, OR 97351, USA.
Genes (Basel). 2021 Feb 4;12(2):228. doi: 10.3390/genes12020228.
The regulation of cannabinoid synthesis in is of increasing research interest as restrictions around the globe loosen to allow the plant's legal cultivation. Of the major cannabinoids, the regulation of cannabigerolic acid (CBGA) production is the least understood. The purpose of this study was to elucidate the inheritance of CBGA dominance in and describe a marker related to this chemotype. We produced two crossing populations, one between a CBGA dominant cultivar and a tetrahydrocannabinolic acid (THCA) dominant cultivar, and one between a CBGA dominant cultivar and a cannabidiolic acid (CBDA) cultivar. Chemical and genotyping analyses confirmed that CBGA dominance is inherited as a single recessive gene, potentially governed by a non-functioning allelic variant of the THCA synthase. The "null" THCAS synthase contains a single nucleotide polymorphism (SNP) that may render the synthase unable to convert CBGA to THCA leading to the accumulation of CBGA. This SNP can be reliably used as a molecular marker for CBGA dominance in the selection and breeding of .
植物大麻素合成的调控越来越受到关注，因为全球范围内的限制正在放宽，以允许合法种植该植物。在主要大麻素中，对大麻萜酚酸（CBGA）生产的调控了解最少。本研究旨在阐明 中 CBGA 优势的遗传规律，并描述与这种化学型相关的标记。我们产生了两个杂交群体，一个是 CBGA 优势品种和四氢大麻酚酸（THCA）优势品种之间的杂交，另一个是 CBGA 优势品种和大麻二酚酸（CBDA）品种之间的杂交。化学和基因分型分析证实，CBGA 优势是由单个隐性基因遗传的，可能由 THCA 合酶的非功能等位基因变异体控制。“无效”THCAS 合酶包含一个单核苷酸多态性（SNP），可能使合酶无法将 CBGA 转化为 THCA，导致 CBGA 积累。这个 SNP 可以作为 CBGA 优势的分子标记，用于 在选择和培育 时。
