单体红色荧光蛋白作为天蓝色链霉菌中大分子定位的报告分子。

Monomeric red fluorescent protein as a reporter for macromolecular localization in Streptomyces coelicolor.

作者信息

Nguyen Khoa Duy, Au-Young Stephanie H, Nodwell Justin R

机构信息

Department of Biochemistry and Biomedical Sciences, McMaster University, Health Sciences Centre, 1200 Main Street West, Hamilton, Ont., Canada L8N 3Z5.

出版信息

Plasmid. 2007 Sep;58(2):167-73. doi: 10.1016/j.plasmid.2007.03.005. Epub 2007 Jun 4.

DOI:10.1016/j.plasmid.2007.03.005

PMID:17544507

Abstract

The enhanced green fluorescent protein (eGFP) is widely used to investigate cell type specific gene expression and protein localization in the filamentous streptomycetes. To broaden the scope of cell biological investigation in these organisms, we have adapted shuttle vectors for the construction of gene fusions to the monomeric red fluorescent protein (mRFP1) and have tested them in Streptomyces coelicolor. Using fusions of mRFP1 to the cell division proteins DivIVA and FtsZ, we show that mRFP1 is comparable to eGFP for cell biological research in this organism and suggest that this paves the way for the future use of two-color imaging and FRET.

摘要

增强型绿色荧光蛋白（eGFP）被广泛用于研究丝状链霉菌中细胞类型特异性基因表达和蛋白质定位。为了拓宽这些生物体中细胞生物学研究的范围，我们改造了穿梭载体，用于构建与单体红色荧光蛋白（mRFP1）的基因融合体，并在天蓝色链霉菌中进行了测试。通过将mRFP1与细胞分裂蛋白DivIVA和FtsZ融合，我们表明mRFP1在该生物体的细胞生物学研究中与eGFP相当，并表明这为未来双色成像和荧光共振能量转移（FRET）的应用铺平了道路。