Bertotto A, Castellucci G, Scalise F, Vaccaro R
Istituto di Clinica Pediatrica, Università degli Studi di Perugia, Italia.
Pediatr Med Chir. 1991 Jul-Aug;13(4):325-9.
Recent studies in our laboratory have demonstrated that the great majority of human colostral T cells display the phenotypic and functional characteristics of memory T lymphocytes, e.g. were able to proliferate in response to anti-CD3 and anti-CD2 monoclonal antibodies, and to a lesser extent, to the lectin mitogen phytohaemagglutinin. In addition, their production of interferon-gamma after anti-CD3 and anti-CD2 stimuli was similar to that calculated in autologous blood lymphocyte cultures. More interestingly, the proportion of T lymphocytes bearing the gamma/delta T-cell receptor was found to be significantly higher in the mammary secretion than in autologous and heterologous blood samples. Furthermore, these cells were mostly delta-TCS-1+, thereby suggesting that they are actively motile cells capable of migrating from lymphoid to extra-lymphoid body tissues. The fact that the phenotypic pattern of colostral gamma/delta T cells is similar, if not identical, to that of the intestinal intraepithelial counterpart suggests that these cells might originate in the gut-associated lymphoid system and home selectively to the mammary gland late in pregnancy and throughout lactation. However, additional studies are needed to confirm whether milk T lymphocytes are actively involved in the adoptive lactation transmission of cellular immunity to the suckling infant.
我们实验室最近的研究表明，绝大多数人初乳中的T细胞表现出记忆性T淋巴细胞的表型和功能特征，例如能够对抗CD3和抗CD2单克隆抗体产生增殖反应，对凝集素丝裂原植物血凝素的反应程度较低。此外，它们在抗CD3和抗CD2刺激后产生γ-干扰素的情况与自体血淋巴细胞培养中的计算结果相似。更有趣的是，发现乳腺分泌物中携带γ/δ T细胞受体的T淋巴细胞比例明显高于自体和异体血样本。此外，这些细胞大多为δ-TCS-1+，这表明它们是能够从淋巴组织迁移到淋巴外身体组织的活跃运动细胞。初乳中γ/δ T细胞的表型模式即使不完全相同，也与肠道上皮内对应细胞相似，这一事实表明这些细胞可能起源于肠道相关淋巴系统，并在妊娠后期和整个哺乳期选择性地归巢到乳腺。然而，需要进一步的研究来证实乳汁中的T淋巴细胞是否积极参与了细胞免疫向哺乳婴儿的过继性乳汁传递。
