Reed W, Erichsen A, Roald B
Department of Pathology, Aker Hospital, Oslo, Norway.
Pathol Res Pract. 1991 Sep;187(7):824-7. doi: 10.1016/S0344-0338(11)80578-9.
The preservation of morphological details in frozen sections subjected to microwave-assisted fixation was compared with that in frozen sections subjected to snap fixation with either formalin/ethanol or ethanol alone. Especially nuclear details were better preserved after short microwave fixation. Ethanol alone gave the poorest results, with loss of nuclear details in the majority of the cases. In this study there was no significant difference between the morphological results obtained with a cheap domestic oven and that with a specialized microwave processor. Microwave-assisted fixation in frozen sections is a simple, rapid method. It is recommended for use in routine laboratories.
将微波辅助固定的冰冻切片与用福尔马林/乙醇或仅用乙醇进行快速固定的冰冻切片的形态学细节保存情况进行了比较。短时间微波固定后，尤其是细胞核细节保存得更好。仅用乙醇的效果最差，大多数情况下细胞核细节会丢失。在本研究中，使用廉价家用烤箱和专用微波处理器获得的形态学结果之间没有显著差异。冰冻切片的微波辅助固定是一种简单、快速的方法。推荐在常规实验室中使用。
