Choi Jung Min, Lim Hye Seong, Kim Jeong Joo, Song Ok-Kyu, Cho Yunje
National Creative Initiatives for Structural Biology, Pohang University of Science and Technology, Pohang, Kyung Book 790-784, South Korea.
Genes Dev. 2007 Jun 1;21(11):1316-21. doi: 10.1101/gad.1548107.
The GINS complex mediates the assembly of the MCM2-7 (minichromosome maintenance) complex with proteins in a replisome progression complex. The eukaryotic GINS complex is composed of Sld5, Psf1, Psf2, and Psf3, which must be assembled for cell proliferation. We determined the crystal structure of the human GINS complex: GINS forms an elliptical shape with a small central channel. The structures of Sld5 and Psf2 resemble those of Psf1 and Psf3, respectively. In addition, the N-terminal and C-terminal domains of Sld5/Psf1 are permuted in Psf2/Psf3, which suggests that the four proteins have evolved from a common ancestor. Using a structure-based mutational analysis, we identified the functionally critical surface regions of the GINS complex.
GINS复合物介导MCM2-7（微小染色体维持）复合物与复制体前进复合物中的蛋白质组装。真核生物的GINS复合物由Sld5、Psf1、Psf2和Psf3组成，其组装对于细胞增殖是必需的。我们确定了人GINS复合物的晶体结构：GINS形成一个带有小中央通道的椭圆形。Sld5和Psf2的结构分别类似于Psf1和Psf3的结构。此外，Sld5/Psf1的N端和C端结构域在Psf2/Psf3中发生了置换，这表明这四种蛋白质是由一个共同祖先进化而来的。通过基于结构的突变分析，我们确定了GINS复合物的功能关键表面区域。
