GINS是古细菌DNA复制叉中的一个核心枢纽。
GINS, a central nexus in the archaeal DNA replication fork.
作者信息
Marinsek Nina, Barry Elizabeth R, Makarova Kira S, Dionne Isabelle, Koonin Eugene V, Bell Stephen D
机构信息
MRC Cancer Cell Unit, Hutchison MRC Research Centre, Hills Road, Cambridge CB2 2XZ, UK.
出版信息
EMBO Rep. 2006 May;7(5):539-45. doi: 10.1038/sj.embor.7400649. Epub 2006 Feb 17.
Abstract
In eukaryotes, the GINS complex is essential for DNA replication and has been implicated as having a role at the replication fork. This complex consists of four paralogous GINS subunits, Psf1, Psf2, Psf3 and Sld5. Here, we identify an archaeal GINS homologue as a direct interaction partner of the MCM helicase. The core archaeal GINS complex contains two subunits that are poorly conserved homologues of the eukaryotic GINS subunits, in complex with a protein containing a domain homologous to the DNA-binding domain of bacterial RecJ. Interaction studies show that archaeal GINS interacts directly with the heterodimeric core primase. Our data suggest that GINS is important in coordinating the architecture of the replication fork and provide a mechanism to couple progression of the MCM helicase on the leading strand with priming events on the lagging strand.
摘要
在真核生物中，GINS复合物对于DNA复制至关重要，并且被认为在复制叉处发挥作用。该复合物由四个同源的GINS亚基Psf1、Psf2、Psf3和Sld5组成。在这里，我们鉴定出一种古细菌GINS同源物作为MCM解旋酶的直接相互作用伙伴。核心古细菌GINS复合物包含两个亚基，它们是真核生物GINS亚基的同源物，保守性较差，与一种含有与细菌RecJ的DNA结合结构域同源结构域的蛋白质形成复合物。相互作用研究表明，古细菌GINS直接与异二聚体核心引发酶相互作用。我们的数据表明，GINS在协调复制叉的结构方面很重要，并提供了一种机制，将前导链上MCM解旋酶的进展与滞后链上的引发事件联系起来。
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