McGarvey Kelly M, Greene Eriko, Fahrner Jill A, Jenuwein Thomas, Baylin Stephen B
The Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University, Baltimore, MD 21231, USA.
Cancer Res. 2007 Jun 1;67(11):5097-102. doi: 10.1158/0008-5472.CAN-06-2029.
Recent work suggests a link between the polycomb group protein EZH2 and mediation of gene silencing in association with maintenance of DNA methylation. However, we show that whereas basally expressed target cancer genes with minimal DNA methylation have increased transcription during EZH2 knockdown, densely DNA hypermethylated and silenced genes retain their methylation and remain transcriptionally silent. These results suggest that EZH2 can modulate transcription of basally expressed genes but not silent genes that are densely DNA methylated.
近期研究表明，多梳蛋白家族成员EZH2与基因沉默介导以及DNA甲基化维持之间存在联系。然而，我们发现，在EZH2敲低过程中，那些DNA甲基化程度最低的基础表达的靶癌基因转录增加，而DNA高度甲基化且沉默的基因则保持其甲基化状态并维持转录沉默。这些结果表明，EZH2可调节基础表达基因的转录，但对DNA高度甲基化的沉默基因则无此作用。
