Dilernia Dario A, Lourtau Leonardo, Gomez Alejandro M, Ebenrstejin Juan, Toibaro Javier J, Bautista Christian T, Marone Rubén, Carobene Mauricio, Pampuro Sandra, Gomez-Carrillo Manuel, Losso Marcelo H, Salomón Horacio
National Reference Center for AIDS, School of Medicine, University of Buenos Aires, Buenos Aires, Argentina.
AIDS. 2007 Jun 19;21(10):1355-60. doi: 10.1097/QAD.0b013e3280b07db1.
Our objective was to estimate primary resistance in an urban setting in a developing country with a long history of antiretroviral delivery and high coverage levels.
We carried out a resistance surveillance study according to WHO HIV-Resistance Guidelines.
Blood samples were collected from 323 drug-naive HIV-1 infected individuals diagnosed at two HIV voluntary counselling and testing centers in Buenos Aires. Viral-load, CD4 cell counts and detuned assays were performed on all samples. The pol gene was sequenced and the resistance profile determined. Phylogenetic analysis was performed by neighbor-joining trees and bootscanning analysis.
We found that 12 (4.2%) of the 284 samples sequenced harbored primary resistance mutations, of which K103N, M41L and V108I were most prevalent. Phylogenetic analysis revealed evidence for the transmission of the K103N mutation among the drug-naive population. The proportion of recent infections identified by the detuned assay was 10.1%.
Levels of primary resistance in Buenos Aires are still low, despite a long history of ARV delivery and high coverage levels.
我们的目的是在一个有着长期抗逆转录病毒治疗历史且覆盖率高的发展中国家城市环境中估计原发性耐药情况。
我们根据世界卫生组织的艾滋病毒耐药指南开展了一项耐药监测研究。
从布宜诺斯艾利斯的两个艾滋病毒自愿咨询和检测中心诊断出的323名未接受过抗逆转录病毒治疗的艾滋病毒-1感染者中采集血样。对所有样本进行病毒载量、CD4细胞计数和失谐检测。对pol基因进行测序并确定耐药谱。通过邻接法树和靴带扫描分析进行系统发育分析。
我们发现，在测序的284个样本中，有12个（4.2%）携带原发性耐药突变，其中K103N、M41L和V108I最为常见。系统发育分析揭示了K103N突变在未接受过抗逆转录病毒治疗人群中传播的证据。失谐检测确定的近期感染比例为10.1%。
尽管有长期的抗逆转录病毒治疗历史且覆盖率高，但布宜诺斯艾利斯的原发性耐药水平仍然较低。
