Montagna Paola, Capellino Silvia, Villaggio Barbara, Remorgida Valentino, Ragni Nicola, Cutolo Maurizio, Ferrero Simone
Laboratory and Division of Rheumatology, Department of Internal Medicine, University of Genoa, Genoa, Italy.
Fertil Steril. 2008 Jul;90(1):156-64. doi: 10.1016/j.fertnstert.2006.11.200. Epub 2007 Jun 4.
To investigate the expression of estrogen receptors (ERs) and inflammatory cytokines in macrophages obtained from peritoneal fluid (PF) of women with endometriosis.
Comparative immunocytochemical study.
University hospital.
PATIENT(S): Thirty women with endometriosis and 22 controls.
INTERVENTION(S): The PF samples were collected at laparoscopy.
MAIN OUTCOME MEASURE(S): The expression of ERalpha, ERbeta, differentiation markers (CD68, NCL-MACRO, HAM56), and inflammatory cytokines (interleukin [IL]-1beta, tumor necrosis factor-alpha [TNF-alpha], IL-6) in PF macrophages was determined.
RESULT(S): The expression of CD68, NCL-MACRO, HAM56, TNFalpha, IL-6, and IL-1beta was significantly higher in PF macrophages obtained from women with endometriosis than in controls. The ERalpha and ERbeta had significantly higher expression in macrophages of women with endometriosis than in controls. A positive correlation was observed between the expression of ERalpha and ERbeta both in women with and without endometriosis. The ERalpha expression was positively correlated with the expression of inflammatory cytokines in women with endometriosis but not in controls; ERbeta expression was correlated to the expression of inflammatory cytokines in the both groups.
CONCLUSION(S): There is a correlation between the expression of ERbeta and proinflammatory cytokines both in women with and without endometriosis. The expression of ERalpha correlates with cytokine production selectively in women with endometriosis but not in controls.
研究子宫内膜异位症患者腹腔液（PF）中巨噬细胞雌激素受体（ERs）和炎性细胞因子的表达情况。
比较性免疫细胞化学研究。
大学医院。
30例子宫内膜异位症患者和22例对照者。
在腹腔镜检查时采集PF样本。
测定PF巨噬细胞中ERα、ERβ、分化标志物（CD68、NCL-MACRO、HAM56）和炎性细胞因子（白细胞介素[IL]-1β、肿瘤坏死因子-α[TNF-α]、IL-6）的表达。
子宫内膜异位症患者PF巨噬细胞中CD68、NCL-MACRO、HAM56、TNFα、IL-6和IL-1β的表达显著高于对照组。子宫内膜异位症患者巨噬细胞中ERα和ERβ的表达显著高于对照组。在有和没有子宫内膜异位症的女性中，均观察到ERα和ERβ表达之间呈正相关。在子宫内膜异位症患者中，ERα表达与炎性细胞因子表达呈正相关，而在对照组中无此相关性；ERβ表达在两组中均与炎性细胞因子表达相关。
在有和没有子宫内膜异位症的女性中，ERβ表达与促炎细胞因子之间均存在相关性。ERα表达仅在子宫内膜异位症患者中与细胞因子产生相关，而在对照组中无此相关性。
