Su Chunxia, Duan Xiangguo, Wang Xiuqing, Wang Chen, Cao Rubing, Zhou Bin, Chen Puyan
Key Laboratory of Animal Diseases Diagnosis & Immunology of China's Department of Agriculture, Nanjing Agricultural University, Jiangsu, Nanjing 210095, PR China.
Vet Microbiol. 2007 Oct 6;124(3-4):256-63. doi: 10.1016/j.vetmic.2007.04.030. Epub 2007 Apr 27.
Mycobacterium tuberculosis heat shock protein70 (HSP70) is a major antigen with both chaperone and cytokine functions. It has been used as an adjuvant to induce or potentiate humoral and cellular immunity, both in the form of a mixture with peptide antigens, and as a fusion protein. We have evaluated the effects of HSP70 on foot and mouth virus (FMDV) subunit vaccines. FMDV VP1, and a synthetic multi-epitope FMDV (EG), and VP1-HSP70 and EG-HSP70 fusion proteins were all heterologously expressed in the yeast Pichia pastoris, and used as antigen in mice. The recombinant VP1 and EG alone was able to induce both humoral and marginal cell-mediated immune responses, while the HSP70 fusions markedly enhanced both the humoral and cell-mediated immune responses. The most prominent immune responses arose from vaccination with the EG-HSP70 fusion product. Both fusion protein-induced Th1-like cytokine (IFN-gamma) and Th2-like cytokine (IL-4) were identified.
结核分枝杆菌热休克蛋白70（HSP70）是一种兼具伴侣蛋白和细胞因子功能的主要抗原。它已被用作佐剂，以与肽抗原混合的形式以及作为融合蛋白来诱导或增强体液免疫和细胞免疫。我们评估了HSP70对口蹄疫病毒（FMDV）亚单位疫苗的影响。FMDV VP1、合成多表位FMDV（EG）以及VP1-HSP70和EG-HSP70融合蛋白均在酵母毕赤酵母中异源表达，并用作小鼠抗原。单独的重组VP1和EG能够诱导体液免疫和边缘细胞介导的免疫反应，而HSP70融合蛋白则显著增强了体液免疫和细胞介导的免疫反应。最显著的免疫反应来自用EG-HSP70融合产物进行的疫苗接种。鉴定出了两种融合蛋白诱导的Th1样细胞因子（IFN-γ）和Th2样细胞因子（IL-4）。
