采用光谱法研究大黄素与DNA在溴化乙锭存在下的相互作用。
Studies of interaction of emodin and DNA in the presence of ethidium bromide by spectroscopic method.
作者信息
Bi Shuyun, Zhang Hanqi, Qiao Chunyu, Sun Ying, Liu Chunming
机构信息
College of Chemistry, Changchun Normal University, Changchun 130032, People's Republic of China.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2008 Jan;69(1):123-9. doi: 10.1016/j.saa.2007.03.017. Epub 2007 Mar 18.
Emodin interacting with deoxyribonucleic acid (DNA) has been studied by different spectroscopic techniques, such as fluorescence, ultraviolet and visible (UV-vis), and fourier transform infared (FT-IR) spectroscopies, using ethidium bromide (EB) as a fluorescence probe of DNA. The decrease in the fluorescence of DNA-EB system on addition of emodin shows that the fluorescence quenching of DNA-EB complex by emodin occurs. The binding constants of emodin with DNA in the presence of EB are 6.02x10(4), 9.20x10(4) and 1.17x10(5)Lmol(-1) at 20, 35 and 50 degrees C, respectively. FT-IR spectrum further suggests that both the phosphate groups and the bases of DNA react with emodin. The reaction of DNA with emodin in the presence of EB is affected by ionic strength and temperature. The values of melting temperature (T(m)) of DNA-EB complex and emodin-DNA-EB complexes were determined, respectively. From the experiment evidences, the major binding mode of emodin with DNA should be the groove binding.
已通过不同的光谱技术研究了大黄素与脱氧核糖核酸（DNA）的相互作用，例如荧光光谱、紫外可见（UV-vis）光谱和傅里叶变换红外（FT-IR）光谱，使用溴化乙锭（EB）作为DNA的荧光探针。加入大黄素后DNA-EB体系荧光的降低表明大黄素对DNA-EB复合物发生了荧光猝灭。在20、35和50℃时，大黄素在EB存在下与DNA的结合常数分别为6.02×10⁴、9.20×10⁴和1.17×10⁵L·mol⁻¹。FT-IR光谱进一步表明DNA的磷酸基团和碱基均与大黄素发生反应。在EB存在下DNA与大黄素的反应受离子强度和温度的影响。分别测定了DNA-EB复合物和大黄素-DNA-EB复合物的解链温度（T(m)）值。根据实验证据，大黄素与DNA的主要结合模式应为沟槽结合。
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