Zhou Guo-Xiong, Huang Jie-Fei, Zhang Hong, Chen Jian-Ping
Department of Gastroenterology, Affiliated Hospital of Nantong University, Nantong 226001, China.
Hepatobiliary Pancreat Dis Int. 2007 Jun;6(3):308-11.
Telomerase activity is reported to be specific and frequent in human pancreatic cancer. We conducted this study to assess the usefulness of monitoring telomerase activity in exfoliated cells obtained by pancreatic duct brushing during endoscopic retrograde cholangiopancreatography (ERCP) for the diagnosis of pancreatic cancer.
Exfoliated cells obtained by pancreatic duct brushing during ERCP from 21 patients (18 with pancreatic cancer, 3 with chronic pancreatitis) were examined. Telomerase activity was detected by polymerase chain reaction and telomeric repeat amplification protocol assay (PCR-TRAP-ELISA).
D450 values of telomerase activity were 0.446+/-0.2700 in pancreatic cancer and 0.041+/-0.0111 in chronic pancreatitis. 77.8% (14/18) of patients with pancreatic cancer had cells with telomerase activity. None of the samples from patients with chronic pancreatitis showed telomerase activity, when the cutoff value of telomerase activity was set at 2.0. Cytological examination showed cancer cells in 66.7% (12/18) of the patients.
Telomerase activity may be an early malignant event in pancreatic cancer development. Cytology and telomerase activity in cells obtained by pancreatic duct brushing may complement each other for the diagnosis of pancreatic cancer.
据报道，端粒酶活性在人类胰腺癌中具有特异性且较为常见。我们开展这项研究，以评估在内镜逆行胰胆管造影术（ERCP）期间通过胰管刷检获取的脱落细胞中端粒酶活性监测对胰腺癌诊断的实用性。
对21例患者（18例胰腺癌患者，3例慢性胰腺炎患者）在ERCP期间通过胰管刷检获取的脱落细胞进行检查。通过聚合酶链反应和端粒重复序列扩增协议分析（PCR - TRAP - ELISA）检测端粒酶活性。
胰腺癌中端粒酶活性的D450值为0.446±0.2700，慢性胰腺炎中为0.041±0.0111。77.8%（14/18）的胰腺癌患者细胞具有端粒酶活性。当端粒酶活性的临界值设定为2.0时，慢性胰腺炎患者的样本均未显示端粒酶活性。细胞学检查显示66.7%（12/18）的患者存在癌细胞。
端粒酶活性可能是胰腺癌发生过程中的早期恶性事件。胰管刷检获取的细胞中的细胞学检查和端粒酶活性检查可能在胰腺癌诊断中相互补充。
