Jontell M, Jiang W H, Bergenholtz G
Department of Endodontology/Oral Diagnosis, Faculty of Odontology, University of Göteborg, Sweden.
Scand J Dent Res. 1991 Oct;99(5):384-9. doi: 10.1111/j.1600-0722.1991.tb01045.x.
Class II antigen expressing cells are generally associated with the early phase of the immune response. Dendritic cells and macrophages expressing these cell surface antigens have recently been demonstrated and characterized in the dental pulp. The present study was undertaken to determine when the pulp receives its immunologic defense potential by examining the temporal appearance of class II antigen expressing cells in the rat incisor pulp. Pulp tissue specimens obtained at various time periods from a gestational age of 16 days to 14 wk after birth were examined by immunohistochemistry using O x 6 as a primary antibody and the ABC-technique. Comparisons were made with tissue samples from the spleen, intestine, skin and oral mucosa. At birth, all tissues, except for the pulp, presented cells expressing class II antigen with a dendritic appearance in a number and orientation resembling the mature tissue. A complete distribution of these cells was not seen in the dental pulp until 7 weeks following birth. Data show that the dental pulp acquires its ultimate structural arrangement of immune cells later than other tissues.
II类抗原表达细胞通常与免疫反应的早期阶段相关。最近在牙髓中已证实并鉴定出表达这些细胞表面抗原的树突状细胞和巨噬细胞。本研究旨在通过检查大鼠切牙髓中II类抗原表达细胞的出现时间，来确定牙髓何时获得其免疫防御潜能。使用Ox 6作为一抗并采用ABC技术，通过免疫组织化学对从出生后16天胎龄至14周不同时间段获取的牙髓组织标本进行检查。并与来自脾脏、肠道、皮肤和口腔黏膜的组织样本进行比较。出生时，除牙髓外，所有组织均呈现出表达II类抗原的细胞，其数量和取向类似成熟组织的树突状外观。直到出生后7周，牙髓中才见到这些细胞的完整分布。数据表明，牙髓获得其免疫细胞的最终结构排列比其他组织要晚。
