Bergenholtz G, Nagaoka S, Jontell M
Department of Endodontology and Oral Diagnosis, Faculty of Odontology, University of Gothenburg, Sweden.
Int Endod J. 1991 Jan;24(1):8-14. doi: 10.1111/j.1365-2591.1991.tb00864.x.
This study reports on the occurrence of class II antigen expressing cells in inflammatory lesions experimentally induced in the rat incisor pulp. Concanavalin A and lipopolysaccharide of Bacteroides gingivalis were applied to the exposed pulp following a preparation through alveolar bone and dental tissues in the midpart of the root. In a set of control teeth, pulpal exposures were capped with Cavit without the placement of antigenic material. Animals were killed after 3, 12, 24, 48 or 96 hours. Pulp tissue specimens were subjected to immunohistochemical analysis utilizing a monoclonal mouse anti-rat class II antigen antibody. Semi-quantitative assessment of positively stained cells was carried out under the light microscope. Significantly more class II antigen expressing cells were identified in the challenged pulps than in the controls at all experimental periods. The increase in cells peaked at 48 hours to taper off at the subsequent 96-hour observation. The rapid and intense influx of class II antigen expressing cells suggests that these cells are associated with the initial defence of the dental pulp.
本研究报告了在大鼠切牙髓中实验性诱导的炎性病变中II类抗原表达细胞的出现情况。在通过牙槽骨和牙根中部的牙组织进行制备后，将刀豆球蛋白A和牙龈拟杆菌的脂多糖应用于暴露的牙髓。在一组对照牙中，牙髓暴露处以Cavit覆盖，不放置抗原物质。在3、12、24、48或96小时后处死动物。牙髓组织标本用单克隆小鼠抗大鼠II类抗原抗体进行免疫组织化学分析。在光学显微镜下对阳性染色细胞进行半定量评估。在所有实验阶段，在受攻击的牙髓中鉴定出的II类抗原表达细胞明显多于对照组。细胞数量的增加在48小时达到峰值，随后在96小时观察时逐渐减少。II类抗原表达细胞的快速和强烈涌入表明这些细胞与牙髓的初始防御有关。
