丁烷氧化为丁醇并与NAD⁺/NADH的电化学氧化还原反应相偶联。
Oxidation of butane to butanol coupled to electrochemical redox reaction of NAD+/NADH.
作者信息
Kang Hye Sun, Na Byung Kwan, Park Doo Hyun
机构信息
Department of Biological Engineering, Seokyeong University, Jungneung-dong 16-1, Sungbuk-gu, Seoul, 136-704, Korea.
出版信息
Biotechnol Lett. 2007 Aug;29(8):1277-80. doi: 10.1007/s10529-007-9385-7. Epub 2007 Jun 5.
A crude cell extract from a butane-utilizing bacterium, Alcaligenes sp., catalyzed the oxidation of butane to butanol coupled to NADH. A graphite electrode modified with Neutral Red (NR-electrode) catalyzed the reduction of NAD(+) to NADH. About 4.9 mM butanol was produced from 50% n-butane/O(2) mixture through the combined reactions of the crude enzyme and the NR-electrode in 250 ml reactor for 3 h.
来自利用丁烷的细菌产碱杆菌属的粗细胞提取物催化丁烷氧化为丁醇并与NADH偶联。用中性红修饰的石墨电极（NR电极）催化NAD(+)还原为NADH。在250毫升反应器中，通过粗酶和NR电极的联合反应，50%正丁烷/O(2)混合物在3小时内产生了约4.9毫摩尔的丁醇。
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