Huberman L, Gollop N, Mumcuoglu K Y, Breuer E, Bhusare S R, Shai Y, Galun R
Department of Food Sciences, Volcani Center, ARO, Beit Dagan, Israel.
Med Vet Entomol. 2007 Jun;21(2):127-31. doi: 10.1111/j.1365-2915.2007.00668.x.
Low molecular weight compounds were isolated by high-performance liquid chromatography from the maggot or haemolymph extracts of Lucilia sericata (Meigen) (Diptera: Calliphoridae). Using gas chromatography-mass spectrometry analysis, three compounds were obtained: p-hydroxybenzoic acid (molecular weight 138 Da), p-hydroxyphenylacetic acid (molecular weight 152 Da) and octahydro-dipyrrolo[1,2-a;1',2'-d] pyrazine-5,10-dione (molecular weight 194 Da), also known as the cyclic dimer of proline (or proline diketopiperazine or cyclo[Pro,Pro]). All three molecules revealed antibacterial activity when tested against Micrococcus luteus and/or Pseudomonas aeruginosa, and the effect was even more pronounced when these molecules were tested in combination and caused lysis of these bacteria.
通过高效液相色谱法从丝光绿蝇（Meigen）（双翅目：丽蝇科）的蛆或血淋巴提取物中分离出低分子量化合物。利用气相色谱 - 质谱分析，获得了三种化合物：对羟基苯甲酸（分子量138道尔顿）、对羟基苯乙酸（分子量152道尔顿）和八氢 - 二吡咯并[1,2 - a;1',2'-d]吡嗪 - 5,10 - 二酮（分子量194道尔顿），也称为脯氨酸的环状二聚体（或脯氨酸二酮哌嗪或环[脯氨酸，脯氨酸]）。当针对藤黄微球菌和/或铜绿假单胞菌进行测试时，所有这三种分子均显示出抗菌活性，并且当这些分子联合测试时效果更为显著，并导致这些细菌裂解。
