School of Natural and Applied Sciences, University of Lincoln, Brayford Pool, Lincoln, LN6 7TS, UK.
J Microbiol Methods. 2010 Sep;82(3):234-7. doi: 10.1016/j.mimet.2010.06.005. Epub 2010 Jun 21.
Research to quantify the potency of larval excretion/secretion from Lucilia sericata using liquid culture assays has produced contradictory results. In this study, viable counting was used to investigate the effectiveness of excretion/secretion against three marker bacterial species (Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli) and the effects of varying growing conditions in assays. Results demonstrate that factors such as number of larvae, species of bacteria and addition of nutrient influence its antibacterial potency. Therefore a standardised method should be employed for liquid culture assays when investigating the antibacterial activity of larval excretion/secretion from L. sericata.
使用液体培养测定法来量化丝光绿蝇幼虫排泄物/分泌物的效力的研究产生了相互矛盾的结果。在这项研究中，使用活菌计数来研究排泄物/分泌物对三种标记细菌（铜绿假单胞菌、金黄色葡萄球菌和大肠杆菌）的效果，以及在测定中不同生长条件的影响。结果表明，幼虫数量、细菌种类和添加营养等因素都会影响其抗菌效力。因此，在研究丝光绿蝇幼虫排泄物/分泌物的抗菌活性时，应该采用标准化方法进行液体培养测定。
