Kengne P, Antonio-Nkondjio C, Awono-Ambene H P, Simard F, Awolola T S, Fontenille D
Research Unit 016, Institute of Research for Development (Unité de Recherche 016, Institut de Recherche pour le Développement [IRD]), Montpellier, France.
Med Vet Entomol. 2007 Jun;21(2):177-82. doi: 10.1111/j.1365-2915.2007.00681.x.
Distinction between members of the equatorial Africa malaria vector Anopheles moucheti (Evans) s.l. (Diptera: Culicidae) has been based mainly on doubtful morphological features. To determine the level of genetic differentiation between the three morphological forms of this complex, we investigated molecular polymorphism in the gene encoding for mitochondrial cytochrome oxidase b (CytB) and in the ribosomal internal transcribed spacers (ITS1 and ITS2). The three genomic regions revealed sequence differences between the three morphological forms similar in degree to the differences shown previously for members of other anopheline species groups or complexes (genetic distance d = 0.047-0.05 for CytB, 0.084-0.166 for ITS1 and 0.03-0.05 for ITS2). Using sequence variation in the ITS1 region, we set up a diagnostic polymerase chain reaction (PCR) for rapid and reliable identification of each subspecies within the An. moucheti complex. Specimens of An. moucheti s.l. collected in Cameroon, the Democratic Republic of Congo (DRC), Uganda and Nigeria were successfully identified, demonstrating the general applicability of this technique.
赤道非洲疟疾媒介莫氏按蚊（Evans）复合组（双翅目：蚊科）成员之间的区分主要基于可疑的形态特征。为了确定该复合组三种形态型之间的遗传分化水平，我们研究了线粒体细胞色素氧化酶b（CytB）编码基因以及核糖体内部转录间隔区（ITS1和ITS2）的分子多态性。这三个基因组区域显示出三种形态型之间的序列差异，其程度与先前其他按蚊物种组或复合组成员所显示的差异相似（CytB的遗传距离d = 0.047 - 0.05，ITS1的遗传距离d = 0.084 - 0.166，ITS2的遗传距离d = 0.03 - 0.05）。利用ITS1区域的序列变异，我们建立了一种诊断性聚合酶链反应（PCR），用于快速可靠地鉴定莫氏按蚊复合组内的每个亚种。在喀麦隆、刚果民主共和国、乌干达和尼日利亚采集的莫氏按蚊复合组标本均被成功鉴定，证明了该技术的普遍适用性。
